| 河朔荛花农药生物活性初探(英文) |
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| 引用本文： | 杨华,田锐,陈宏力. 河朔荛花农药生物活性初探(英文)[J]. （《农业科学与技术》）编辑部, 2009, 0(1) |
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| 作者姓名： | 杨华 田锐 陈宏力 |
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| 作者单位： | 延安大学化工学院; |
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| 基金项目： | Supported by School Project of Yan’an University (YD2005-042)~~ |
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| 摘 要： | [Objective] The insecticidal and antibacterial bioactivity of Wikstroemia chamaedaphne Meissn were screened and bioactive substances in it were separated and purified. [Method]The Wikstroemia chamaedaphne Meissn was conducted ultrasonic extraction in petroleum ether, ethyl acetate and methanol. The insecticidal activity of Wikstroemia chamaedaphne Meissn to Mythimna separata walker and aphid were determined. The antibacterial activity of Wikstroemia chamaedaphne Meissn to Fusarium graminearu, Glomerella cingulata, F.oxysporium f.sp niveum, Alternaria solani and Fusarium oxysporium were also determined. The bioactivity-guided methods such as opencolumn chromatography and Pre-HPLC method were used to separate active components in petroleum ether extract from Wikstroemia chamaedaphne Meissn. [Result] When the concentration was 500 mg/L, 3 kinds of extracts from Wikstroemia chamaedaphne Meissn didn’t show obvious antibacterial bioactivity to 5 kinds of test samples. When the concentration was 5%, petroleum ether extract show certain topical toxicity to aphids. The ethyl acetate extract showed certain antifeedant activity to 3rd instar Larvae of Mythimna separata Walker. The fraction F4 of petroleum ether extract possessed highest topical toxicity to aphids and the lethality was 60.00%.[Conclusion] Wikstroemia chamaedaphne Meissn contained many insecticidal constituents whose active parts and mechanism were needed further researches.
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| 关 键 词： | 生物活性 粘虫 乙酸乙酯提取物 蚜虫 番茄早疫病菌 石油醚提取物 活性测定 小麦赤霉病菌 西瓜枯萎病菌 河朔荛花 |
A Preliminary Study on Biological Activity of Wikstroemia chamaedaphne Meissn |
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| Abstract: | [Objective] The insecticidal and antibacterial bioactivity of Wikstroemia chamaedaphne Meissn were screened and bioactive substances in it were separated and purified. [Method]The Wikstroemia chamaedaphne Meissn was conducted ultrasonic extraction in petroleum ether, ethyl acetate and methanol. The insecticidal activity of Wikstroemia chamaedaphne Meissn to Mythimna separata walker and aphid were determined. The antibacterial activity of Wikstroemia chamaedaphne Meissn to Fusarium graminearu, Glomerella cin... |
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| Keywords: | Wikstroemia chamaedaphne Meissn Insecticidal activity Separation of active components |
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