| 转双价基因抗虫杨抑制美国白蛾的作用机理研究 |
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| 引用本文： | 杨利艳,孙毅,崔贵梅.转双价基因抗虫杨抑制美国白蛾的作用机理研究[J].华北农学报,2008,23(5). |
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| 作者姓名： | 杨利艳 孙毅 崔贵梅 |
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| 作者单位： | 1. 山西大学,生物技术研究所,山西,太原,030006;山西省农业生物技术研究中心,山西,太原,030031;山西师范大学,生命科学学院,山西,临汾,041004
2. 山西省农业生物技术研究中心,山西,太原,030031 |
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| 摘 要： | 为明确双价基因chitinase-BmkIT对鳞翅目害虫的作用机制,以美国白蛾为供试虫源,用转双价基因(烟草天蛾几丁质酶基因和东亚钳蝎昆虫特异性神经毒素基因)杨树叶片饲喂其幼虫,至3龄时,采用石蜡切片法,对美国白蛾幼虫中肠结构进行观察,并对其头部乙酰胆碱酯酶活性进行测定.结果表明,取食转基因杨树叶片的美国白蛾幼虫中肠结构不完整,中肠细胞出现排列不整齐、脱落等现象;而取食非转化杨树叶片的美国白蛾幼虫中肠结构完整,中肠细胞排列整齐紧密.转基因杨树叶片还显著抑制了美国白蛾幼虫头部乙酰胆碱酯酶的活性(p＜0.05).取食转chitinase-BmkIT基因杨树叶片抑制了幼虫的生长,并使部分幼虫身体溃烂.该双价基因可作为防治美国白蛾的基因源.
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| 关 键 词： | 美国白蛾 几丁质酶基因 蝎昆虫神经毒素基因 中肠 乙酰胆碱酯酶 |
Investigation on Inhibition Mechanism of Transgenic Poplar with Binary Insectresistant Genes to Hyphantria cunea |
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| YANG Li-yan,SUN Yi,CUI Gui-mei.Investigation on Inhibition Mechanism of Transgenic Poplar with Binary Insectresistant Genes to Hyphantria cunea[J].Acta Agriculturae Boreali-Sinica,2008,23(5). |
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| Authors: | YANG Li-yan SUN Yi CUI Gui-mei |
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| Institution: | YANG Li-yan1,2,3,SUN Yi2,CUI Gui-mei2 (1.Biotechnology Institute,Shanxi University,Taiyuan 030006,China,2.The Agri-Biotechnology Research Centre of Shanxi Province,Taiyuan 030031,3.College of Life Science,Shanxi Normal University,Linfen 041004,China) |
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| Abstract: | To reveal the mechanism of binary genes chitinase-BmkIT on Lepidopterous insect,larvae of Hyphantria cunea were fed with the leaves of transgenic poplar harboring chitinase gene and BmkIT gene.The 3rd instar larvae midgut structures were observed and the acetylcholinesterase activities in their heads were measured.The results showed that midgut structures of larvae fed with transgenic leaves were incomplete and cells were disordered and detached versus those fed with transformed control.At the same time,tra... |
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| Keywords: | Hyphantria cunea Chitinase gene BmkIT gene Midgut Acetylcholinesterase |
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