Establishment and characterization of dengue virus type 2 nonstructural protein 1 specific T cell lines |
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Authors: | Yang Xiao-meng Jiang Li-fang Tang Yun-xia Yin Yue Liu Wen-quan Fang Dan-yun |
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Institution: | a Department of Microbiology, Zhongshan School of Medicine, Sun Yat-Sen University, 74#, Zhongshan 2nd Road, Guangzhou, 510080, China |
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Abstract: | Immunity against dengue viruses (DENV) infection may include cellular immune responses which involve in the immunopathology of DENV infection hosts. This study was to establish short-term dengue virus type 2 (DENV2) nonstructural protein 1 (NS1) specific T cells from splenocytes from BALB/c mice immunized with DENV2 NS1 in vitro, which may be used to identify immunopathologic mechanism of dengue. Nine DENV2 NS1 specific T cell lines were successfully established by using limiting dilution methods and maintained for 20 weeks by re-stimulated with DENV2 NS1, recombinant mouse IL-2 and antigen presenting cell weekly. Phenotypically, these cells were mainly composed of CD3+CD4+ T cells. The culture supernatants of these cells contained large amounts of TNF-α and IFN-γ. Vascular tissue pathological change could be found in the mice adoptive transferred with DENV2 NS1 specific T cells. The results indicate that DENV2 NS1 specific T cells could be established and maintained with syngeneic T cell growth factors in vitro. Meanwhile, DENV2 NS1 specific T cells might contribute to the immunopathology of vascular leakage of dengue. |
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Keywords: | Dengue virus type 2 Nonstructural protein 1 Antigen-specific T cells |
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