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| 昆虫生长调节剂和绿僵菌对意大利蝗几丁质酶活力的影响 | |
| 引用本文： | 赵忠伟,曹广春,徐光青,吴乐年,张泽华.昆虫生长调节剂和绿僵菌对意大利蝗几丁质酶活力的影响[J].植物保护,2012,38(2):83-86. |
| 作者姓名： | 赵忠伟 曹广春 徐光青 吴乐年 张泽华 |
| 作者单位： | 1. 中国农业科学院植物保护研究所,农业部生物防治重点开放实验室,北京100081;沈阳农业大学植物保护学院,沈阳110161 2. 中国农业科学院植物保护研究所,农业部生物防治重点开放实验室,北京100081 3. 新疆阿勒泰地区治蝗灭鼠办公室,阿勒泰,836500 4. 新疆哈巴河县治蝗办公室,哈巴河,836700 |
| 基金项目： | 公益性行业(农业)科研专项(20090321,201003079);农业部现代农业专项(nycytx-2009-37) |
| 摘 要： | 目的]探明3种昆虫生长调节剂(苯氧威、氟虫脲、灭幼脲)和一种生物农药(绿僵菌189菌株)对意大利蝗3龄蝗蝻几丁质酶活力的影响.方法]用药剂处理3龄意大利蝗蝗蝻,用分光光度法测定几丁质酶分解几丁质产物N-乙酰氨基葡萄糖的含量.结果]3种昆虫生长调节剂浓度达到50mg/L时对几丁质酶的抑制作用超过200％;绿僵菌189菌株对意大利蝗几丁质酶活力也有一定程度的抑制,且抑制作用随着孢子浓度的增加而增大,当孢子浓度达到5×107时,意大利蝗几丁质酶的活力仅为3.8U.结论]昆虫生长调节剂和绿僵菌均抑制意大利蝗几丁质酶活力. |
| 关 键 词： | 意大利蝗 昆虫生长调节剂 绿僵菌 几丁质酶 |
The effect of insect growth regulators and Metarhizium anisopliae on the Calliptamus italicus chitinase |
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| Zhao Zhongwei,Cao Guangchun,Xu Guangqing,Wu Lenian,Zhang Zehua.The effect of insect growth regulators and Metarhizium anisopliae on the Calliptamus italicus chitinase[J].Plant Protection,2012,38(2):83-86. | |
| Authors: | Zhao Zhongwei Cao Guangchun Xu Guangqing Wu Lenian Zhang Zehua |
| Institution: | 1 (1.Key Laboratory for Biological Control of Ministry of Agriculture,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100081,China; 2.College of Plant Protection,Shenyang Agricultural University,Shenyang 110161,China; 3.Control Office of Locust and Rodents,Department of Animal Husbandry of Aletai,Xinjiang 836500,China; 4.Habahe Station of Forecast and Control of Locust and Rodents,Xinjiang 836700,China) |
| Abstract: | Objective] The effect of three insect growth regulators(fenoxycarb,flufenoxuron,diflubenzuron) and a biological pesticide(189 strains of Metarhizium anisopliae) on the chitinase of the 3rd instars of Calliptamus italicus was studied.Method] The third instars of the C.italicus were treated by three pesticides and M.anisopliae,respectively.The content of N-acetyl glucosamine produced by chitin decomposition with chitinase was tested by spectrophotometer.Result] The results showed that all of the four pesticides inhibited chitinase activity.When the concentration of each of the three insect growth regulators reached to 50mg/L,their inhibitory effects on the chitinases were more than 200%.189 strains of M.anisopliae had some inhibitory effects on chitinase of C.italicus.When the spores reached the highest concentration of 5 × 107,the chitinase activity had only 3.8U.Conclusion] Both insect growth regulators and M.anisopliae can inhibite the chitinase activity of C.italicus. |
| Keywords: | Calliptamus italicus insect growth regulator Metarhizium anisopliae chitinase |
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