| 二氟沙星残留检测的Ci-ELISA方法建立 |
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| 引用本文： | 潘孝成,祁克宗,朱良强,赵瑞宏.二氟沙星残留检测的Ci-ELISA方法建立[J].中国畜牧兽医,2007,34(10):127-129. |
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| 作者姓名： | 潘孝成 祁克宗 朱良强 赵瑞宏 |
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| 作者单位： | 1.安徽省农科院畜牧兽医所，合肥 230031；2.安徽农业大学，合肥 230036；3.安徽省兽医站,合肥 230022 |
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| 摘 要： | 以合成的二氟沙星（DIF）人工抗原为基础，制备出了高效价抗DIF血清（1∶29×104），并建立了DIF间接竞争酶联免疫吸附测定法(Ci-ELISA法)。标准曲线的线性回归方程为y=-0.223x+0.9631(r=0.9929)，中值（I50）=119.21 ng/ml，最低检测限（LOD）=1.14 ng/ml，线性范围为1.14~10000 ng/ml，方法的平均批内变异系数（CV）为4.83%,平均批间CV为10.69%。DIF以浓度15~10000 ng/ml在鸡肉中添加时，回收率为81.24%~90.64%，变异系数为8.18%~11.75%。
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| 关 键 词： | 二氟沙星 间接竞争酶联免疫吸附测定法 残留 鸡肉 |
| 文章编号： | 1671-7236（2007）10-0127-03 |
| 修稿时间： | 2007-03-29 |
EstabLishment of Indirect Competitive Enzyme-linked Immunosorbent Assay for Difloxacin |
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| PAN Xiao-cheng,QI Ke-zong,ZHU Liang-qiang,ZHAO Rui-hong.EstabLishment of Indirect Competitive Enzyme-linked Immunosorbent Assay for Difloxacin[J].China Animal Husbandry & Veterinary Medicine,2007,34(10):127-129. |
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| Authors: | PAN Xiao-cheng QI Ke-zong ZHU Liang-qiang ZHAO Rui-hong |
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| Institution: | 1.Institute of Animal and Veterinary Science, Anhui Academy of Agricultural Science, Hefei 230031, China;2.Anhui Agricultural University, Hefei 230036,China;3.Anhui Veterinary Station,Hefei 230022,China |
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| Abstract: | The Ci ELISA method for the detection of difloxacin were established with the polyclonal antibodies against difloxacin. The regression equation, the 50% inhibition concentration(IC50), the limits of detect (LOD) and the linear range about the standard curve of difloxacin were y=-0.223x+0.9631(r=0.9929), 119.21ng/ml，1.14 ng/ml and 1.14~10000 ng/ml, respectively. The average coefficients of variation for the Ci ELISA method were 4.83% in intra assay and 10.69% in inter assay. The recoveries in chicken ranged from 81.24% to 90.64%. |
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| Keywords: | difloxacin Ci-ELISA residue chicken |
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