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细胞外环境中分化抑制物对动物胚胎干细胞克隆率的影响
引用本文:安立龙,效梅,窦忠英,邱怀.细胞外环境中分化抑制物对动物胚胎干细胞克隆率的影响[J].家畜生态学报,2001,22(2):44-49.
作者姓名:安立龙  效梅  窦忠英  邱怀
作者单位:西北农林科技大学畜牧兽医学院,
基金项目:国家自然科学基金资助(批准号:39670359),国家攻关课题资助(编号96-C0103),校青年教师专项基金资助。
摘    要:介绍了饲养层、条件培养基、分化抑制因子对哺乳动物胚胎干细胞分离与克隆的影响,并探讨了白血病抑制因子影响胚胎干细胞克隆的机理。

关 键 词:胚胎干细胞  饲养层  条件培养基  克隆
文章编号:1004-5228(2001)02-0044-06
修稿时间:2000年10月31

The Influence of Differentiation Inhibitor in Extracellular Environment on Clone of Embryonic Stem Cell in Mammal Animals
AN Li-long,XIAO Mei,DOU Zhong-ying,QIU Huai.The Influence of Differentiation Inhibitor in Extracellular Environment on Clone of Embryonic Stem Cell in Mammal Animals[J].Acta Ecologae Animalis Domastici,2001,22(2):44-49.
Authors:AN Li-long  XIAO Mei  DOU Zhong-ying  QIU Huai
Abstract:The influence of feeder layer, conditioned medium and differentiation inhibitory activity on isolation and clone of embryonic stem cell were introduced and the physiological mechanism for differentiation inhibitory activity influencing on isolation and embryonic stem cell in mammal animals was discussed in this paper .
Keywords:Embryonic Stem cell  feeder layer  Conditioned medium  differentiation inhibitory activity  clone
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