农杆菌介导的丹参遗传转化体系研究进展

丹参是我国传统的大宗药材,被广泛用于治疗心脑血管疾病。随着我国心脑血管疾病发病率的逐年攀升,丹参的种质资源也急需有新的提高,转基因技术作为基因功能研究和转基因育种的重要手段在丰富和优化丹参种质资源方面取得了显著成效。通过利用转基因技术中常用的农杆菌介导法来转入外源基因以及探求丹参有效成分合成代谢相关基因功能,以期获得高产、高抗丹参植株的方法已较为成熟,目前研究工作主要集中于不同遗传转化体系的建立和优化。本文就丹参遗传转化体系构建的转化原理、影响因素和研究现状三方面作出详细综述,为今后丹参基因功能研究和新品种培育提供一定参考。

Research progress on the genetic transformation system of Salvia miltiorrhiza mediated by Agrobacterium

Salvia is a traditional medicinal material in China and is widely used in the treatment of cardiovascular and cerebrovascular diseases.With the increasing incidence of cardiovascular and cerebrovascular diseases in our country, the germplasm resources of Salvia miltiorrhiza are also in urgent need of new improvement.As an important means of gene function research and transgenic breeding, transgenic technology has achieved remarkable results in enriching and optimizing the germplasm resources of Salvia miltiorrhiza.By using the Agrobacterium-mediated method commonly used in transgenic technology to transfer exogenous genes and explore the functions of genes related to the synthesis and metabolism of Salvia miltiorrhiza active components, the method of obtaining high-yield and high-resistance Salvia miltiorrhiza plants is relatively mature. The current research work mainly focuses on different Establishment and optimization of genetic transformation systems.In this paper, a detailed review of the transformation principle, influencing factors and research status of the genetic transformation system of Salvia miltiorrhiza is made, which will provide a certain reference for the future research on the gene function of Salvia miltiorrhiza and the cultivation of new varieties.