| 一个与马铃薯青枯病抗性连锁的RAPD标记 |
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| 引用本文： | 郜刚,屈冬玉,连勇,金黎平,冯兰香.一个与马铃薯青枯病抗性连锁的RAPD标记[J].植物病理学报,2002,32(3):267-271. |
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| 作者姓名： | 郜刚 屈冬玉 连勇 金黎平 冯兰香 |
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| 作者单位： | 1 山西师范大学生物系, 临汾 041004;2 中国农科院蔬菜花卉研究所, 北京 100081 |
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| 摘 要： | 用原始栽培种Solanum phureja作为抗源构建了一个二倍体马铃薯作图群体,并且用于进行群分法(bulked segregant analysis,BSA)分析,以筛选检定马铃薯青枯病抗性的RAPD连锁标记。使用300个随机引物进行RAPD检测,发现引物OPG09可在抗感性DNA池之间产生960 bp的稳定的相斥型多态性产物。进一步分析分离群体并得到与抗性相连锁的标记OPG09960。该标记已有效地应用于检测其它具相近遗传背景的二倍体群体的抗性。
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| 关 键 词： | 马铃薯 青枯病 抗性 RAPD |
A RAPD MARKER LINKED TO THE POTATO RESISTANCE TO BACTERIAL WILT |
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| Abstract.A RAPD MARKER LINKED TO THE POTATO RESISTANCE TO BACTERIAL WILT[J].Acta Phytopathologica Sinica,2002,32(3):267-271. |
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| Authors: | Abstract |
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| Institution: | 1 Department of Biology, Shanxi Teachers University, Linfen 041004;2 Institute of Vegetables and Flowers, CAAS, Beijing 100081, China |
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| Abstract: | A highly resistant primitive cultivated species Solanum phureja was employed to generate a mapping population to perform the bulked segregant analysis (BSA) for screening and study of RAPD markers linked to the potato resistance to bacterial wilt. The random amplified polymorphic DNA (RAPD) analysis was made to identify polymorphic markers between resistant bulk and susceptible bulk DNA with 300 random primers. Primer OPG09 produced a 960 bp reproducible band only in the resistant clones, linking to the resistance in the population. The marker was successfully used in examination of another population with similar genetic background. |
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| Keywords: | potato bacterial wilt resistance RAPD |
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