| 蚕蛹不同溶解性蛋白的营养学评价及酶解物对血管紧张素转换酶的抑制活性 |
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| 引用本文： | 吴琼英,徐金玲,贾俊强,桂仲争,谭广秀. 蚕蛹不同溶解性蛋白的营养学评价及酶解物对血管紧张素转换酶的抑制活性[J]. 蚕业科学, 2011, 37(2) |
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| 作者姓名： | 吴琼英 徐金玲 贾俊强 桂仲争 谭广秀 |
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| 作者单位： | 江苏科技大学生物与环境工程学院,江苏镇江,212018;中国农业科学院蚕业研究所,江苏镇江,212018;江苏科技大学生物与环境工程学院,江苏镇江,212018;中国农业科学院蚕业研究所,江苏镇江,212018 |
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| 基金项目： | 江苏省"六大人才高峰"第七批资助项目，江苏科技大学青年骨干教师支持计划项目 |
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| 摘 要： | 分别对缫丝蚕蛹中的水溶性和碱溶性蛋白组分进行营养学评价,为高效开发蚕蛹食品提供依据;通过蚕蛹蛋白酶解产物对血管紧张素转换酶(ACE)的体外抑制活性试验,探讨蚕蛹蛋白作为天然降压药品原料的利用潜力。营养学分析表明:蚕蛹蛋白水溶性和碱溶性组分中的氨基酸种类齐全,且必需氨基酸占总氨基酸的质量分数分别为41.2%和39.2%,明显高于WHO推荐的氨基酸组成模式(>36%);蚕蛹水溶性蛋白有第1～第4限制性氨基酸(依次为亮氨酸、异亮氨酸、缬氨酸和苏氨酸),而蚕蛹碱溶性蛋白仅有第1和第2限制性氨基酸(依次为蛋氨酸+胱氨酸、异亮氨酸),2种溶解性蛋白的生物效价均较低,在开发利用中,应补充限制性氨基酸或与其它蛋白搭配使用,以提高产品的营养价值。体外ACE抑制活性试验表明,蚕蛹的水溶性蛋白和碱溶性蛋白的碱性蛋白酶酶解产物均具有较强的ACE抑制活性,IC50值分别为0.121、0.113 mg/mL,2种蛋白有可能成为降血压肽生产原料。
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| 关 键 词： | 蚕蛹 水溶性蛋白 碱溶性蛋白 氨基酸种类 血管紧张素转换酶 体外抑制 |
Nutrition Evaluation of Silkworm Pupal Proteins with Various Solubility and Inhibitory Activity of Their Enzymatic Digested Products to Angiotensin-converting Enzyme |
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| WU Qiong-Ying,XU Jin-Ling,JIA Jun-Qiang,GUI Zhong-Zheng,TAN Guang-Xiu. Nutrition Evaluation of Silkworm Pupal Proteins with Various Solubility and Inhibitory Activity of Their Enzymatic Digested Products to Angiotensin-converting Enzyme[J]. Acta Sericologica Sinica, 2011, 37(2) |
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| Authors: | WU Qiong-Ying XU Jin-Ling JIA Jun-Qiang GUI Zhong-Zheng TAN Guang-Xiu |
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| Affiliation: | WU Qiong-Ying1 XU Jin-Ling1 JIA Jun-Qiang2 GUI Zhong-Zheng1,2 TAN Guang-Xiu1(1School of Biotechnology and Environmental Engineering,Jiangsu University of Science and Technology,Zhenjiang Jiangsu 212018,China,2The Sericultural Research Institute,Chinese Academy of Agricultural Sciences,China) |
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| Abstract: | In order to provide reference for efficient development of food with silkworm pupae,the water-soluble and alkali-soluble protein fractions from silkworm pupae of reeled cocoons were evaluated on their nutritional quality.Their utilization potential as protein resources for preparing natural blood pressure reducing medicine was investigated through assaying the in vitro inhibition activity of their alcalase hydrolysates to angiotensin-converting enzyme(ACE).Nutrition analysis showed that both water-soluble a... |
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| Keywords: | Silkworm pupa Water-soluble protein Alkali-soluble protein Amino acid variety Angiotensin-converting enzyme(ACE) In vitro inhibition |
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