| Ac/Ds转座子系统及其在玉米突变体库构建中的应用 |
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| 引用本文： | 董雷,库丽霞,陈彦惠,李平华.Ac/Ds转座子系统及其在玉米突变体库构建中的应用[J].玉米科学,2015,23(2):1-6. |
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| 作者姓名： | 董雷 库丽霞 陈彦惠 李平华 |
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| 作者单位： | 河南农业大学/河南粮食作物协同创新中心, 郑州450002,河南农业大学/河南粮食作物协同创新中心, 郑州450002,河南农业大学/河南粮食作物协同创新中心, 郑州450002,河南农业大学/河南粮食作物协同创新中心, 郑州450002;山东农业大学农学院, 山东泰安271018 |
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| 摘 要： | Ac/Ds转座子系统是研究玉米功能基因组学的重要工具。Ac/Ds转座子系统以其在基因组中的低拷贝、倾向插入基因外显子、可以在同一基因不同位点造成突变等独特优势,在构建玉米突变体库和进行基因功能鉴定方面具有重要应用前景。综述了Ac/Ds转座子系统的插入特点、转座机制、在玉米突变体库构建中的应用以及我国玉米突变体库的现状,并对我国玉米Ac/Ds突变体库的研究提出展望。
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| 关 键 词： | 玉米 Ac/Ds转座子 突变体库 |
| 收稿时间： | 8/8/2014 12:00:00 AM |
Ac/Ds Transposon System and Its Utilization in Mutant Library Construction in Maize |
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| DONG Lei,KU Li-xi,CHEN Yan-hui and LI Ping-hua.Ac/Ds Transposon System and Its Utilization in Mutant Library Construction in Maize[J].Journal of Maize Sciences,2015,23(2):1-6. |
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| Authors: | DONG Lei KU Li-xi CHEN Yan-hui and LI Ping-hua |
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| Institution: | Collaborative Innovation Center of Henan Grain Crops, Henan Agricultural University, Zhengzhou 450002,Collaborative Innovation Center of Henan Grain Crops, Henan Agricultural University, Zhengzhou 450002,Collaborative Innovation Center of Henan Grain Crops, Henan Agricultural University, Zhengzhou 450002 and Collaborative Innovation Center of Henan Grain Crops, Henan Agricultural University, Zhengzhou 450002;Agronomy College of Shandong Agricultural University, Tai'an 271018, China |
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| Abstract: | Ac/Ds transposon system is an important tool for maize functional genomics. Its distinctive properties, e.g. low copy number in the genome, insertion basis to the exons, and mutating different loci in a single gene, make it a wonderful system for mutant library construction and gene function characterization in maize. The specificity and mechanisms of Ac/Ds transposon in maize as well as the application of this system in constructing the mutant library were reviewed. The current progress of mutant library construction and further utilization of Ac/Ds in China was also discussed. |
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| Keywords: | Maize Ac/Ds transposon Mutant library |
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