| 对玉米螟具有生物活性的海洋放线菌筛选与鉴定 |
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| 引用本文： | 刘志航,袁忠林,罗兰. 对玉米螟具有生物活性的海洋放线菌筛选与鉴定[J]. 玉米科学, 2015, 23(2): 147-151 |
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| 作者姓名： | 刘志航 袁忠林 罗兰 |
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| 作者单位： | 青岛农业大学农学与植物保护学院/山东省植物病虫害综合防控重点实验室, 山东青岛266109,青岛农业大学农学与植物保护学院/山东省植物病虫害综合防控重点实验室, 山东青岛266109,青岛农业大学农学与植物保护学院/山东省植物病虫害综合防控重点实验室, 山东青岛266109 |
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| 基金项目： | 国家级大学生创新创业训练计划(201210435002)、“十二五”农村领域国家科技计划课题(BAD19B04-09)、山东省高等学校青年骨干教师国内访问学者项目、山东省“泰山学者”建设工程专项、青岛农业大学第六届大学生创新教育立项经费 |
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| 摘 要： | 通过平板稀释法对来自青岛不同地区的海洋样品进行分离,共得到18株海洋放线菌。采用浸虫法测定海洋放线菌发酵液对玉米螟2龄幼虫的室内毒力,筛选得到2株活性较高的菌株YC3和SLR3,其48 h发酵液对玉米螟2龄幼虫的校正死亡率分别为86.21%和81.04%。根据形态特征、培养特征及生理生化性状将活性菌株鉴定为链霉菌属(Streptomyces)。
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| 关 键 词： | 玉米 放线菌 发酵液 玉米螟 |
| 收稿时间： | 2014-06-12 |
Screening and Identification of Marine Actinomyces with Bioactive for Asian Corn Borer(Ostrinia furnacalis G.) |
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| LIU Zhi-hang,YUAN Zhong-lin and LUO Lan. Screening and Identification of Marine Actinomyces with Bioactive for Asian Corn Borer(Ostrinia furnacalis G.)[J]. Journal of Maize Sciences, 2015, 23(2): 147-151 |
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| Authors: | LIU Zhi-hang YUAN Zhong-lin LUO Lan |
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| Affiliation: | College of Agronomy and Plant Protection of Qingdao Agricultural University/Key Lab of Integrated Crop Pest Management of Shandong Province, Qingdao 266109, China,College of Agronomy and Plant Protection of Qingdao Agricultural University/Key Lab of Integrated Crop Pest Management of Shandong Province, Qingdao 266109, China and College of Agronomy and Plant Protection of Qingdao Agricultural University/Key Lab of Integrated Crop Pest Management of Shandong Province, Qingdao 266109, China |
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| Abstract: | Eighteen strain marine actinomyces were isolated from sea samples of Qingdao different regions by using dilution-plate method. Toxicities of fermentation broth from these marine actinomyces were assayed against 2 instar larvae of Asian corn borer by insect immersion bioassay method, and two strains had insecticidal activities for Ostrinia furnacalis G. The corrected mortality rates were 86.21% and 81.04% respectively in 48 h after treatment with supernatant from fermentation broth of YC3 and SLR3. The morphological and cultural characteristics, physiological and biochemical properties showed two strains were Streptomyces. |
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| Keywords: | Maize Actinomyces Fermentation broth Ostrinia furnacalis G. |
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