| 加拿大进境大豆上检出菜豆荚斑驳病毒 |
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| 引用本文： | 沈建国,王念武,翁瑞泉,黄可辉,郭琼霞. 加拿大进境大豆上检出菜豆荚斑驳病毒[J]. 植物保护, 2009, 35(6): 127-129. DOI: 10.3969/j.issn.0529-1542.2009.06.028 |
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| 作者姓名： | 沈建国 王念武 翁瑞泉 黄可辉 郭琼霞 |
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| 作者单位： | 福建出入境检验检疫局,福建省检验检疫技术研究重点实验室,福州,350001 |
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| 基金项目： | 福建省自然科学基金项目，福建出入境检验检疫局科技项目，福建科技重大专项专题项目 |
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| 摘 要： | 本研究采用DAS ELISA、IC RT PCR及序列测定方法，对加拿大进境大豆种子进行菜豆荚斑驳病毒（Bean pod mottle virus, BPMV）和大豆花叶病毒（Soybean mosaic virus,SMV）检测，结果表明，BPMV的DAS ELISA和IC RT PCR检测结果均为阳性，且PCR扩增产物序列与已报道的BPMV基因序列相似性达97%以上，而SMV的DAS ELISA和IC RT PCR检测结果都为阴性。综合血清学、分子生物学检测结果，确认该批大豆携带有菜豆荚斑驳病毒。
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| 关 键 词： | 菜豆荚斑驳病毒 检测 DAS ELISA IC RT PCR |
Detection of Bean pod mottle virus in soybean imported from Canada |
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| Shen Jianguo,Wang Nianwu,Weng Ruiquan,Huang Kehui,Guo Qiongxia. Detection of Bean pod mottle virus in soybean imported from Canada[J]. Plant Protection, 2009, 35(6): 127-129. DOI: 10.3969/j.issn.0529-1542.2009.06.028 |
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| Authors: | Shen Jianguo Wang Nianwu Weng Ruiquan Huang Kehui Guo Qiongxia |
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| Affiliation: | Fujian Exit Entry Inspection and Quarantine Bureau, Fujian Key Laboratory for Technology Research of Inspection and Quarantine, Fuzhou350001, China |
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| Abstract: | The presence of BPMV and SMV in soybean seeds imported from Camda was determined using DASELISA,IC-RT-PCR and sequence analysis.The results showed that both DAS-ELISA and IC-RT-PCR were positive for BPMV,and the nucleotide sequence of BPMV PCR product had more than 97% similarity to previously reported genornic sequence.However,negative DAS-ELISA and IC-RT-PCR results were obtained for SMV.According to the results of serological and molecular biological methods,it was confirmed that BPMV was present in tested soybean imported from Camda. |
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| Keywords: | DAS-ELISA IC-RT-PCR Bean pod mottle virus detection DAS-ELISA IC-RT-PCR |
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