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Identification and activity of ethyl gallate as an antimicrobial compound produced by Geranium carolinianum
Authors:ATSUSHI OOSHIRO  SYUNTARO HIRADATE  SHINJI KAWANO  TETSUYA TAKUSHI  YOSHIHARU FUJII  MASAHIRO NATSUME   HIROSHI ABE
Affiliation:Okinawa Prefectural Agricultural Research Center, Nago,;Okinawa Prefectural Agricultural Research Center, Itoman, Okinawa,;Department of Biological Safety Science, National Institute for Agro-Environmental Science, Tsukuba and;Division of Agriscience and Bioscience, Institute of Symbiotic Science and Technology, Tokyo University of Agriculture and Technology, Tokyo, Japan
Abstract:We isolated an antimicrobial compound from the aerial tissue of Geranium carolinianum and identified it as ethyl 3, 4, 5-trihydroxy benzoate (ethyl gallate) by 1H-NMR and 13C-NMR and gas chromatography–mass spectrometry. The antimicrobial activity of ethyl gallate against three potato pathogens was assayed by the paper disk method. The activity against Ralstonia solanacerum , Streptomyces scabies , and Streptomyces acidiscabies was observed at concentrations >200, >300, and >300 µg disk−1, respectively. These results suggest that the antimicrobial activity of Geranium carolinianum against soil-borne plant disease pathogens is partly related to ethyl gallate.
Keywords:antimicrobial activity    ethyl gallate    Geranium carolinianum L
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