Generation of monoclonal antibodies specific for ORF68 of koi herpesvirus |
| |
| Authors: | Aoki Takashi Takano Tomokazu Unajak Sasimnanas Takagi Madoka Kim Young Rim Park Seong Bin Kondo Hidehiro Hirono Ikuo Saito-Taki Tatsuo Hikima Jun-Ichi Jung Tae Sung |
| |
| Institution: | a Laboratory of Genome Science, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato, Tokyo 108-8477, Japan;b Department of Microbiology, School of Allied Health Science, Kitasato University, Sagamihara, Kanagawa 228-8555, Japan;c Aquatic Biotechnology Center, College of Veterinary Medicine, Gyeongsang National University, 900 Gajwa-dong, Jinju, Gyeongnam 660-710, South Korea |
| |
| Abstract: | Outbreaks of koi herpesvirus (KHV) infection in carp are still a serious problem worldwide. KHV is closely related to other two cyprinid herpesviruses, pox herpesvirus (CHV) and haematopoietic necrosis herpesvirus (CyHV-2) in goldfish. In this study, two major KHV antigenic proteins (ORF62 and ORF68) were identified by immunoscreening using a KHV-specific polyclonal antibody, and then monoclonal antibodies were generated for immunodiagnostic studies. After screening hybridoma cells, one mAb against ORF68 (mAb-7C6) was obtained but no mAbs against ORF62. mAb-7C6 specifically reacted with a lysate of KHV-infected koi fin cells (KF-1 cells) but not with lysates of CHV- or CyHV-2-infected KF-1 cells in an immuno-blotting analysis. Similar results were shown in the following tests: (1) a indirect fluorescent antibody test using infected KF-1 cells and (2) an immunohistochemical investigation by fast red stain (infected liver) or FITC detection (infected spleen). These results suggested that mAb-7C6 specifically reacts with KHV ORF68 protein. |
| |
| Keywords: | Common carp (Cyprinus carpio) Koi herpesvirus (KHV or CyHV-3) CHV (CyHV-1) CyHV-2 Monoclonal antibody (mAb) |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ |
|
