沙冬青提取物(JA1)对小鼠肝癌H22细胞体外增殖和皮下移植生长的抑制

采用噻唑蓝（MTT）还原法测定梯度浓度的沙冬青提取物（JA1）对体外培养小鼠肝癌细胞株H22增殖的影响;同时对皮下移植肿瘤H22小鼠灌服不同剂量的JA1以检测抑瘤率,进一步采用流式细胞术、光镜和透射电子显微镜技术,检测和观察JA1对小鼠移植性肿瘤H22的诱导凋亡作用。结果显示,JA1可显著抑制体外培养小鼠肝癌H22细胞的增殖,并且这种抑制存在浓度和时间关系。同时,JA1对小鼠皮下移植性肿瘤H22也有明显的抑制作用。流式细胞分析表明,JA1灌胃试验组瘤组织细胞DNA直方图上见有明显的凋亡峰;在光镜和电镜下,JA1灌胃试验组也见有明显的瘤细胞凋亡,表明JA1对体内、外H22细胞的增殖抑制是以诱导凋亡为基础的。

Inhibitory effect of JA1 on H22 cells in vitro and in vivo

To investigate the inhibitory effect of the extractive of Ammopiptanthus mongolicus(JA1)on hepatocarcinoma cells and its possible mechanisms,the hepatocellular carcinoma cell line of mice(H22)was used as target cell.The inhibitory effects of JA1 on H22 cells were assayed by MTT in vitro and the antitumor effect of JA1 was also observed in H22-bearing mice by flow cytometry assays,light and transmission electronic microscopy.The results revealed that JA1 had significant anti-proliferative effects on H22 cells with the dependence of time and dosage.In addition,JA1 not only significantly inhibited the proliferation of H22 cells in vitro but also inhibited the growth of H22 hepatocarcinoma in vivo.Flow cytometric analysis indicated that JA1 could induce apoptosis on H22 cells in H22-bearing mice and the G1 phase cell percentage increased and the S phase cell percentage decreased.Morphological change of H22 cells undergoing apoptosis were also detected by light and electron microscopy.These results suggest that JA1 has better inhibitory effects on H22in vitro and vivo and the mechanism maybe related to its apoptosis inducing activity.