血清3型鸭甲型肝炎病毒阳性血清的制备

通过使用血清3型鸭甲型肝炎疫苗免疫SPF鸡,制备了一批血清3型鸭甲型肝炎诊断用阳性血清,并对其进行了无菌、支原体和特异性检验以及中和效价测定。结果表明,该血清无菌检验合格,无支原体污染,特异性良好,无禽类常见13种外源病毒抗体,血清中和效价为10-2.8。本研究为鸭甲型肝炎病毒血清学鉴定及相关研究提供了重要的物质基础。

Preparation of Positive serum against Serum type 3 duck hepatitis A virus

In order to develop a positive serum for the differential diagnosis of duck hepatitis A virus, SPF chickens were inoculated with serum type 3 duck hepatitis vaccine, and a series of tests have been done for the serum acquired form those immune SPF chickens, including sterility test, mycoplasma test, specific test, and neutralization titer test. The results showed that the antiserum was specific for the serum type 3 duck hepatitis A virus, without 13 types of avian common exogenous virus antibody, and free of bacterial and mycoplasma contamination, the neutralization titer was as high as 10-2.8. The research provided material for serological identification and related research of duck hepatitis A virus.