| 墨兰杂交及F1代离体培养研究 |
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| 引用本文： | 左利娟,汤久杨,李志强. 墨兰杂交及F1代离体培养研究[J]. 北京农业职业学院学报, 2017, 31(3): 22-25. DOI: 10.3969/j.issn.1671-7252.2017.03.004 |
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| 作者姓名： | 左利娟 汤久杨 李志强 |
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| 作者单位： | 北京农业职业学院,北京,102442;北京农业职业学院,北京,102442;北京农业职业学院,北京,102442 |
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| 基金项目： | 北京农业职业学院技术研发与推广项目 |
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| 摘 要： | 以墨兰为研究对象,通过人工授粉,检测墨兰与春兰杂交后的坐果率和种子的离体萌发率;种子萌发后形成根状茎,筛选适宜根状茎增殖分化的培养基.结果表明:墨兰为母本的座果率为100%;果龄200~220 d采收适宜于离体播种;离体种子萌发后形成根状茎,其适宜的根状茎增殖分化培养基为1/2MS+6-BA2.0 mg·L-1+KT0.5 mg·L-1+NAA0.05 mg·L-1.
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| 关 键 词： | 墨兰 杂交 离体培养 增殖 |
| 收稿时间： | 2016-11-09 |
Studies on the Hybridization and in Vitro Seed Germination of Cybidium Sinensis |
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| ZUO Li-juan,TANG Jiu-yang,LI Zhi-qiang. Studies on the Hybridization and in Vitro Seed Germination of Cybidium Sinensis[J]. Journal of Beijing Agricultural Vocation College, 2017, 31(3): 22-25. DOI: 10.3969/j.issn.1671-7252.2017.03.004 |
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| Authors: | ZUO Li-juan TANG Jiu-yang LI Zhi-qiang |
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| Affiliation: | Beijing Vocational College of Agriculture, Beijing 102442, China |
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| Abstract: | The artificial pollination test was done between Cybidium sinensis and Cybidium goeringii.The average fruit set and the rate of germination were studied. The results showed that the hybrid rateof Cymbidium sinense×Cymbidium goeringii was 100%; the hybrid capsules with the maturity of200~220ds has the highest germination rate; The appropriate medium was1/2MS+6-BA2.0 mg·L-1+ KT 0.5mg·L-1+ NAA 0.05 mg·L-1 for multiplication and differentiation |
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| Keywords: | Cymbidium sinensis hybridization in vitro germination propagation |
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