华丽牛肝菌菌塘土壤细菌多样性的高通量测序分析

为了解野生华丽牛肝菌(Boletus magnificus)的生境,以华丽牛肝菌菌塘为研究对象,取样4个菌塘土壤,采用高通量测序技术,结合生物信息学方法对4个采集点菌塘土壤进行分析,研究其细菌的组成、丰度及多样性.结果表明,华丽牛肝菌菌塘菌群分属于30个门、78个属,菌塘中主要的类群为变形菌门(72.13％～14.70...

Analysis of Bacterial Diversity in Shiroes Soil of Boletus magnificus by High-throughput Sequencing

Four shiroes soils were collected to investigate the Boletus magnificus habitat and explore the community diversity,community abundance and composition of the soil bacteria. Bacterial diversity of the four shiroes soils were detected by the high-throughput sequencing detection technique and analyzed by the bioinformatics method. Results showed that all of four bacterial community divided into 30 phyla and 78 genera in shiroes bacteria of Boletus magnificus, in which proteobacteria was the dominant flora with a proportion from 72.13% to 14.70%, and followed by Acidobacteria(62.39%-2.97%), Gemmatimonadetes(5.69%-2.22%) and Firmicutes(1.31%-0.05%). The dominant genera of the bacterial community were Burkholderia, Serratia, Sphingomonas, Bradyrhizobium, Gp1, Gp2, Gemmatimonas and WPS-1 genera incertae sedis. The bacterial diversity maintained considerably high relative abundance and excellent diversity index, while the dominant bacteria in the four soil samples were different relying on its growing environment, related to tree species, soil characteristics and human activities in the sampling environment. But all of them contained Burkholderia, Sphingomonas, Bradyrhizobium and Gemmatimonadetes. This study provides a reference for understanding the habitat of wild Boletus.