| 大肠杆菌987 P基因工程菌苗预防实验性仔猪黄痢试验 |
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| 引用本文： | 于涟,王淦,蔡渭明,丁荣法. 大肠杆菌987 P基因工程菌苗预防实验性仔猪黄痢试验[J]. 浙江大学学报(农业与生命科学版), 1992, 0(4) |
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| 作者姓名： | 于涟 王淦 蔡渭明 丁荣法 |
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| 作者单位： | 浙江农业大学畜牧兽医系，中国科学院上海植物生理研究所，浙江农业大学畜牧兽医系，富阳县农业局 杭州 310029，上海 200032，杭州 310029，浙江富阳 311400 |
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| 摘 要： | 用基因工程技术构建的大肠杆菌HB101/pBP52菌株能产生987 P型菌毛,但不产生热稳定肠毒素(ST),用该茵株制备灭活苗,在产前21天免疫18头第一胎怀孕母猪.在分娩后24～48小时内,用产热稳定肠毒素(ST)大肠杆菌987菌株(O9:K103,987P:NM)人工感染所产仔猪102头,88头仔猪在观察期(攻毒后7天)结束时安全无恙,保护率达86.27％.结果表明:用无毒大肠杆菌HB 101/pBP 52菌株制备的灭活苗可以有效地预防987P菌毛型致病性大肠杆菌引起的仔猪黄痢.
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| 关 键 词： | 大肠杆菌疫苗 987 P菌毛 仔猪黄痢 |
Prevention of experimental neonatal colibacillosis with E.coli 987P genetic engineered vaccine. |
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| Yu Lian,WangGan,Cai Weiming,Ding Rongfa. Prevention of experimental neonatal colibacillosis with E.coli 987P genetic engineered vaccine.[J]. Journal of Zhejiang University(Agriculture & Life Sciences), 1992, 0(4) |
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| Authors: | Yu Lian WangGan Cai Weiming Ding Rongfa |
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| Abstract: | Genetic engineered Escherichia coli HB 101/pBP52 strain could produce 987P pili.yet it could not produce heatstable toxin (ST). 18 nulliparous pregnant sows were inoculated with the inactivated vaccine prepared from this strain 21 days before parturition. In 24-48 hours after parturition, 102 heads of their piglets were artificially challenged with ST-producing E. coli 987(O9 : K103 ,987P : NM) ,88 of which were free from any abnormal manifestions within 7 days after challenge with a protection rate of 86. 27%. The results showed that the vaccine made from the strain HB 101/pBP52 could effectively protect piglets from scouring caused by ETEC strain with 987 P pili. |
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| Keywords: | E. coli vaccine 987 P pili neonatal colibacillosis |
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