| 云南不同地区蜘蛛兰上朱顶红褪绿环斑病毒RNA S序列分析 |
| |
| 引用本文： | 王宇琪, 吴保为, 陶宏征, 等. 侵染昆明园林植物的4个HCRV分离株核壳体蛋白氨基酸序列分析[J]. 云南农业大学学报（自然科学）, 2021, 36(1): 165-168. DOI: 10.12101/j.issn.1004-390X(n).201903068 |
| |
| 作者姓名： | 王宇琪 吴保为 陶宏征 贾志强 高雪 王景文 刘雅婷 |
| |
| 作者单位： | 1.云南农业大学 农学与生物技术学院，云南 昆明 650201;2.云南农业大学 植物保护学院，云南 昆明 650201 |
| |
| 基金项目： | 云南省重点项目（2018FA019）；国家自然科学基金项目（31471828，31260451） |
| |
| 摘 要： | 目的比较分析侵染昆明园林植物HCRV核壳体蛋白氨基酸序列，探究地理分布和寄主来源与病毒变异的相关性。方法2016年，在云南省昆明市园林景区采集疑似感染番茄斑萎病毒属(Tospovirus)病毒样品，利用RT-PCR技术测定出葱莲(Zephyranthes candida)、文殊兰(Crinum asiaticum)、喜林芋(Philodendron schott)和酢浆草(Oxalis corniculata) 4份感染朱顶红褪绿环斑病毒(Hippeastrum chlorotic ringspot virus，HCRV)的样品，通过克隆、测序获得4份样品的 S RNA序列，利用ORF Finder获得核壳体蛋白(nucleocapsid protein，N)并进行序列分析。结果文殊兰、喜林芋和酢浆草是首次报道的HCRV新寄主；4个HCRV分离物的N蛋白与NCBI首次报道的HLS1-2 N蛋白(登录号：AFX74694)一致性达到99.34%；系统进化树分析显示：4个HCRV分离物与GenBank公布的同样来自云南的株系聚为1支。结论病毒的地理分布相关性强于寄主相关性。
|
| 关 键 词： | 朱顶红褪绿环斑病毒 核壳体蛋白 序列分析 |
| 收稿时间： | 2019-03-26 |
| 修稿时间： | 2020-11-10 |
A new tospovirus causing chlorotic ringspot on Hippeastrum sp. in China |
| |
| Yuqi WANG, Baowei WU, Hongzheng TAO, et al. Amino Acid Sequence Analysis of Four Isolates of Hippeastrum Chlorotic Ringspot Virus Nucleocapsid Protein in Several Garden Plants in Kunming[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2021, 36(1): 165-168. DOI: 10.12101/j.issn.1004-390X(n).201903068 |
| |
| Authors: | Yuqi WANG Baowei WU Hongzheng TAO Zhiqiang JIA Xue GAO Jingwen WANG Yating LIU |
| |
| Affiliation: | 1.College of Agriculture and Biotechnology, Yunnan Agricultural University, Kunming 650201, China;2.College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China |
| |
| Abstract: | PurposeThe amino acid sequences of HCRV nucleocapsid proteins in Kunming garden plants were compared and analyzed, to explore the viruses variation correlation between geographical distribution and host sources.MethodIn 2016, samples of suspected Tospovirus virus were collected in the garden scenic spot of Kunming City, Yunnan Province. Four samples of HCRV infected with Zephyranthes candida, Crinum asiaticum, Philodendron schott and Oxalis corniculata were determined by RT-PCR. Four samples of S RNA sequences were found by cloning and sequencing and the nucleocapsid (N) protein was obtained using the online software ORF Finder and sequence analysis.ResultC. asiaticum, P. schott and O. corniculata are the first reported new hosts of HCRV. The N protein of the four HCRV isolates and the first reported HLS1-2 N protein of NCBI identity reached 99.34%. The phylogenetic tree shows that the HCRV isolated from Yunnan in this study is clustered with the same strain from Yunnan published by GenBank.ConclusionThe geographical distribution of the virus is stronger than the host correlation. |
| |
| Keywords: | Hippeastrum chlorotic ringspot virus nucleocapsid protein sequence analysis |
|
| 点击此处可从《云南农业大学学报(自然科学版)》浏览原始摘要信息 |
|
点击此处可从《云南农业大学学报(自然科学版)》下载全文 |
