| 茅莓中表儿茶素的含量测定研究 |
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| 引用本文： | 李泽林, 代红丽, 沈晓静, 等. 表儿茶素对急性肺损伤小鼠抗氧化应激和抗炎作用研究[J]. 云南农业大学学报（自然科学）, 2021, 36(5): 848-854. DOI: 10.12101/j.issn.1004-390X(n).202012021 |
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| 作者姓名： | 李泽林 代红丽 沈晓静 付晓萍 冯励 王雪峰 普岳红 范江平 |
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| 作者单位： | 1.云南农业大学 食品科学技术学院，云南 昆明 650201;2.云南农业大学 理学院，云南 昆明 650201 |
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| 基金项目： | 国家自然科学基金项目(31560457)；云南农业大学发展基金项目(KX900002) |
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| 摘 要： | 目的探讨表儿茶素(EC)对脂多糖(LPS)诱导急性肺损伤(ALI)小鼠氧化应激和炎症的调节作用。方法采用气管滴注法建立ALI模型，通过测定肺组织湿干质量比(W/D)评估其水肿程度，检测过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)和超氧化物歧化酶(SOD)的活性以及丙二醛(MDA)和蛋白质羰基的含量以评估其体内抗氧化损伤的作用，检测小鼠血清、支气管肺泡灌洗液(BALF)和肺组织中白细胞介素-1β (IL-1β)、白细胞介素-6 (IL-6)和肿瘤坏死因子-α (TNF-α)的含量、肺组织内一氧化氮(NO)的含量以及磷酸化的P38分裂原激活的蛋白激酶(P38)、细胞外信号调节激酶(ERK)和c-jun N端激酶(JNK)的表达水平评估其抗炎症作用。结果EC (40 mg/kg)预处理能降低ALI小鼠的肺组织的W/D值和水肿程度，提高CAT、GSH-Px和SOD等酶的活性，降低MDA和蛋白质羰基的含量提升其体内抗氧化活性，降低IL-1β、IL-6、TNF-α和NO的含量并降低肺组织p-JNK/JNK (P<0.05)、p-ERK/ERK (P<0.01)和p-P38/P38 (P<0.01)的比值。结论EC可以调节氧化应激和炎症反应改善小鼠的ALI。
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| 关 键 词： | 表儿茶素 脂多糖 急性肺损伤 氧化应激 炎症 |
| 收稿时间： | 2020-12-10 |
| 修稿时间： | 2021-04-09 |
Epicatechin protective effects on bleomycin-induced pulmonary oxidative stress and fibrosis in mice |
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| Zelin LI, Hongli DAI, Xiaojing SHEN, et al. Study on the Anti-oxidative Stress and Anti-inflammatory Effect of Epicatechin on Acute Lung Injury in Mice[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2021, 36(5): 848-854. DOI: 10.12101/j.issn.1004-390X(n).202012021 |
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| Authors: | Zelin LI Hongli DAI Xiaojing SHEN Xiaoping FU Li FENG Xuefeng WANG Yuehong PU Jiangping FAN |
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| Affiliation: | 1.College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China;2.College of Science, Yunnan Agricultural University, Kunming 650201, China |
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| Abstract: | PurposeTo investigate the regulation effect of epicatechin (EC) on lipopolysaccharide (LPS) induced acute lung injury (ALI) mice oxidative stress and inflammation.MethodsA tracheal instillation method was used to establish ALI model in BALB/c mice. The degree of edema of lung tissues were assessed by measuring the wet/dry weight ratio (W/D). Its anti-oxidative damage effects also were detected by evaluating catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) activities, malondialdehyde (MDA) and protein carbonyl content. Serum, bronchoalveolar lavage fluid (BALF) and lung tissues interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and nitric oxide (NO) content and expression of phosphorylated P38 mitogen activated protein kinases (P38), extracellular regulated kinases (ERK) and c-jun N-terminal kinase (JNK) were detected to assess its anti-inflammatory effects.Results EC (40 mg/kg) pretreatment effectively decreased the W/D ratio of lung tissue to improve the degree of edema, and increased the activity of CAT, GSH-Px and SOD and reduced the level of MDA and protein carbonyl to improve its antioxidant activity. EC (40 mg/kg) also reduced the content of IL-1β, IL-6, TNF-α and NO and decreased the ratio of p-JNK/JNK (P<0.05), p-ERK/ERK (P<0.01) and p-P38/P38 (P<0.01) in lung tissues.ConclusionEC can regulate oxidative stress and inflammation and improve ALI in mice. |
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| Keywords: | epicatechin lipopolysaccharide acute lung injury oxidative stress inflammation |
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