中药“扶正固本”与化疗药联合抗肿瘤的研究进展

目的探究铁皮石斛粗多糖对5-氟尿嘧啶(5-FU)诱导引起的小鼠化疗性肠道黏膜炎(CIM)的预防作用。方法健康雄性昆明鼠40只，随机分为正常组、CIM模型组(腹腔注射5-FU 130 mg/kg)及铁皮石斛粗多糖低、中、高剂量组(分别灌胃铁皮石斛粗多糖50、100和200 mg/kg，每日1次，持续2周，24 h后腹腔注射5-FU 130 mg/kg，每日1次，持续2 d)，观察小鼠体质量变化情况，在腹腔注射5-FU 48 h后处死动物，对小肠进行形态学评价，检测小肠增殖细胞核抗原(PCNA)表达，对小肠屏障闭锁小带蛋白1 (ZO-1)、闭锁蛋白(Occludin)、炎症因子白介素1β (IL-1β)和肿瘤坏死因子(TNF-α)表达量进行检测。结果与CIM模型组相比，铁皮石斛粗多糖中剂量(100 mg/kg)组小鼠体质量明显降低；可增加小肠绒毛长度、降低隐窝深度、增加绒毛长度与隐窝深度比值；小肠PCNA表达量明显增加；可提高小肠屏障ZO-1和Occludin mRNA表达量；抑制炎症因子IL-1β和TNF-α mRNA表达量。结论铁皮石斛粗多糖具有预防5-FU引起的化疗性肠道黏膜炎。

Treatment of osteosarcoma around the knee in skeletally immature patients

PurposeTo investigate the preventive effect of Dendrobium officinale polysaccharides on the chemotherapeutic intestinal mucositis (CIM) in mice induced by 5-FU.MethodsForty healthy male Kunming mice were randomly divided into normal group, CIM model group (intraperitoneal injection of 5-FU 130 mg/kg), low, medium, and high-dose groups of D. officinale polysaccharide (50, 100, 200 mg/kg, respectively, once a day for 2 weeks, after 24 hours, mice were intraperitoneally injected with 5-FU 130 mg/kg, once a day, for 2 days). The weight changes of mice were observed. The mice were killed 48 hours after 5-FU was injected intraperitoneally. The morphology of small intestine was evaluated and the expression of proliferating cell nuclear antigen (PCNA) in the small intestine was detected. The expression of ZO-1, Occludin, IL-1β and TNF-α were detected test.ResultsCompared with CIM model group, the middle dose of D. officinale polysaccharide (100 mg/kg) significantly reduced the weight change of mice (P<0.05), increased the villi length, decreased the crypt depth, increased the ratio of villi length to crypt depth, increased the expression of PCNA in small intestine, increased the expression of ZO-1 and Occludin mRNA in intestinal barrier, and inhibited the expression of inflammatory factors IL-1β and TNF-α.ConclusionDendrobium officinale polysaccharides can prevent chemotherapy-induced intestinal mucositis caused by 5-FU.