| 胶体金免疫层析法快速检测水产品中四环素类药物残留 |
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| 引用本文： | 檀尊社,陆 恒,邵 伟,张少恩. 胶体金免疫层析法快速检测水产品中四环素类药物残留[J]. 西北农业学报, 2010, 19(8): 32-7 |
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| 作者姓名： | 檀尊社 陆 恒 邵 伟 张少恩 |
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| 作者单位： | 1. 洛阳市农产品安全检测中心,河南洛阳,471002
2. 杭州南开日新生物技术有限公司,杭州,310022 |
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| 摘 要： | 为建立用于快速检测水产样品中四环素类药物残留含量的胶体金免疫层析检测试剂,采用免疫竞争法,将抗四环素单克隆抗体-胶体金复合物包被在胶体金结合垫上,并将人工合成的四环素抗原包被在硝酸纤维素薄膜表面作为检测线(T线),其残留药物与待测样品中四环素竞争结合胶体金标记的四环素单克隆抗体,最终能以颜色直观显示检测结果。检测水产品试样时,试剂灵敏度最低值可达100 ng/mL,仅需5~10min,与金霉素、土霉素、强力霉素等四环素族抗生素有很强的交叉反应,故可同时检测四环素族中几种主要抗生素,而与沙丁胺醇、莱克多巴胺等其他兽药无交叉反应。试验证明检测试剂具有较高的灵敏度及特异性,操作便捷,稳定可靠,可作为四环素残留现场监控的有效筛检手段。
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| 关 键 词： | 四环素 半抗原 免疫测定 胶体金 免疫层析 |
Nanocolloidal Gold Based Immunoassay for Rapid Detection of Tetracycline Residue in Aquatic Products |
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| TAN Zunshe,LU Heng,SHAO Wei and ZHANG Shaoen. Nanocolloidal Gold Based Immunoassay for Rapid Detection of Tetracycline Residue in Aquatic Products[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2010, 19(8): 32-7 |
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| Authors: | TAN Zunshe LU Heng SHAO Wei ZHANG Shaoen |
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| Affiliation: | TAN Zunshe1,LU Heng1,SHAO Wei2 and ZHANG Shaoen2(1.Luoyang Center for Quality and Safety of Agricultural Products and the System,Luoyang He'nan 471002,China,2.Hangzhou Nankai Biotech Co.,Ltd.,Hangzhou 310022,China) |
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| Abstract: | This study aimed to develop a rapid,simple colloidal gold immunochromatographic assay for detection of tetracycline residue.In this study competitive immunoassay was applied.Colloidal gold combination was coated with anti-tetracycline monoclonal antibody-nanocolloidal gold compound,and nitrocellulose membrane(NC) which covered with man-made tetracycline antigen was made as test line.The drug residue would competitive combined the anti-tetracycline monoclonal antibody with the sample,and the result would be ... |
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| Keywords: | Tetracycline Hapten Immunoassay Gold colloid Immune chromatography |
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