Metal tolerance in barley and wheat cultivars: physiological screening methods and application in phytoremediation

Purpose

The objective of the study was to evaluate the tolerance of barley (Hordeum vulgare) and wheat (Triticum aestivum) in soil contaminated with different concentrations of Cd and Cr, the effect of these metals on some physiological characteristics and the biomass produced in order to assess their potential application in phytoremediation of contaminated soils.

Materials and methods

A greenhouse experiment using two cultivars of barley, CB502 and Pedrezuela, and one of wheat, Albares, was conducted. The pots were watered with different concentrations of Cd or Cr during the period of plant growth. Cellular membrane damage, content and fluorescence of chlorophyll, and plant biomass were evaluated. After harvesting, the content of metals in the roots, shoots and grains was analysed as well as the available metal concentration in soil samples.

Results and discussion

The results show that the Cd treatments did not significantly affect the growth and physiology of the plants, but they were affected by the Cr treatments. At the highest concentration of Cr, the decrease in chlorophyll content compared to the control was 44, 77 and 83 % for the CB502 and Pedrezuela barleys and the Albares wheat respectively. A reduction in biomass was also observed (74, 81 and 85 % respectively). The amount of Cd and Cr accumulated in the root was higher than that accumulated in the aerial part for the three cultivars. The barleys accumulated the highest amount of metal in the roots. The transfer factor was lower in barley than in the wheat for both metals.

Conclusions

Cadmium treatments do not affect plant development. In the case of Cr, plant development and physiological traits were significantly affected. For the highest concentration of Cr, the CB502 barley had the highest tolerance index, 26 %, and the Albares wheat had the lowest one, 15 %. The two barleys were more effective in phytoremediation of soil contaminated with Cd or Cr than the wheat, presenting a higher tolerance to these metals in the assayed conditions.