山羊FSHR基因第10外显子的PCR-SSCP检测及其序列分析

利用PCR-SSCP技术检测山羊(Caprahircus)包括西农萨能奶山羊、关中奶山羊、陕南白山羊、安哥拉山羊和波尔山羊173个个体FSHR基因第10外显子的单核苷酸多态性(SNP)。结果未发现SNP位点。测序后获得山羊FSHR基因第10外显子的核苷酸序列,并在NCBI数据库中获得GenBank登录No.DQ069909和DQ069910。通过DNA序列分析发现,FSHR基因第10外显子第120位碱基不存在C→T的转换,也不存在颠换等其它遗传变化。山羊、绵羊(OvisariesL.)和普通牛(Bostaurus)FSHR基因第10外显子序列同源性比较和聚类分析结果表明,山羊、普通牛和绵羊该部分序列的相似性最高为99.3%;在物种间比较中,绵羊和普通牛纯合子该基因外显子序列的不相似性最高为3.4%;据FSHR基因外显子序列构建的分子系统树结果显示,山羊、绵羊和普通牛物种内的个体各自聚为一类;山羊和绵羊先聚为一类,然后再与普通牛聚为一类。提示FSHR基因第10外显子的核苷酸序列适合于物种间的动物分子树的构建。

PCR-SSCP Detection and DNA Sequence Analysis of Exon 10 of Goat Follicle-stimulating Hormone Receptor (FSHR) Gene

PCR-SSCP was applied to detect single nucleotide polymorphism (SNP) of exon 10 of the follicle-stimulating hormone receptor (FSHR) gene for 173 goats (Capra hircus), including Xinong Sanen Dairy goat, Guangzhong goat, Shaannan White goat, Angera and Boer goat breeds. The results demonstrated monopolymorphism. After sequenced, goat FSHR gene exon10 DNA sequence was firstly acquired ( GenBank Accession No.DQ069909 and DQ069910). DNA sequence analysis showed that molecular mechanism of monopolymorphism was probably due to no nucleotide mutation from C to T or other mutations in 120th nucleotide position. Comparing nucleotide sequences of FSHR gene exon 10 for goat, sheep (Ovisaries L) and bovine (Bos Taurus), the maximal similarity and the minimal divergence was 99.3% and 3.4%, respectively. The molecular phylogenetic tree of goat, sheep and bovine constructed by FSHR gene exon 10 sequences showed that individuals within species were clustered together, respectively; Sheep and goat had the nearest relative relationship, and then clustered with bovine. The result implies that FSHR gene exon 10 sequence is suitable for constructing molecular phylogenetic tree among species.