Analysis of genetic variability in <Emphasis Type="Italic">Couepia</Emphasis> accessions using AFLP markers |
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Authors: | Yomara Rozo Lorena Quintero Mauricio Parra Carlos Rodríguez Luz Marina Melgarejo |
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Institution: | (1) Departamento de Biología, Universidad Nacional de Colombia, Bogota, Colombia;(2) Instituto Amazónico de Investigaciones Científicas SINCHI, Bogota, Colombia;(3) Facultad de Agronomía, Universidad Nacional de Colombia, Bogota, Colombia |
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Abstract: | Couepia is a genus distributed in tropical regions of America. The nut of some Couepia species is used as fresh fruit and oil source for local communities. Despite its critical situation of conservation and its
economic potential, there is a lack of information on the genetic variability of Couepia species. This study examines AFLP variation among 40 accessions of Couepia collected in the Colombian Amazonian region, representing two species: Couepia
dolichopoda and Couepia
subcordata. The individuals were examined for 96 markers generated from four EcoRI/MseI primer pairs, with 80% polymorphism across all accessions studied. According to cluster analysis, 40 accessions were grouped
into two major clusters, corresponding to the two species analyzed, except one case whose situation is discussed. In C. dolichopoda accessions, significant correlation between the clustering pattern and the geographical origin was detected; the extent of
variation within and among its collect sites was examined by AMOVA. The knowledge about the genetic variability of the accessions
examined contributes to development of Couepia conservational efforts. |
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Keywords: | Amazonian resources Chrysobalanaceae Couepia Molecular markers Tropical nut |
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