| 巴西橡胶树胚胎晚期丰富蛋白基因的克隆及序列分析 |
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| 引用本文： | 喻修道,王真辉,袁 坤,杨礼富.巴西橡胶树胚胎晚期丰富蛋白基因的克隆及序列分析[J].热带作物学报,2011,32(7):1304-1308. |
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| 作者姓名： | 喻修道 王真辉 袁 坤 杨礼富 |
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| 作者单位： | 1. 中国热带农业科学院橡胶研究所 海南儋州 571737
2. 农业部橡胶树生物学重点开放实验室 海南儋州 571737 |
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| 基金项目： | 中国热带农业科学院橡胶研究所基本科研业务费专项资金资助项目(No.YWFZX2010-9,No.1630022011014) |
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| 摘 要： | 根据抑制性消减杂交文库分离的EST片段,采用3’-RACE技术从巴西橡胶树胶乳中获得HbLEA14基因的全长cDNA序列,其ORF长456 bp,编码151个氨基酸,预测HbLEA14蛋白分子量为16.58 ku,等电点为5.10。同源性比对显示,HbLEA14与蓖麻RcLEA14、乳浆大戟EeLEA14、陆地棉GhLEA14、大豆GmLEA14、拟南芥AtLEA14、番茄SlER5的同源性分别为88%、80%、75%、73%、65%和61%,属于非典型第2组LEA蛋白。RT-PCR结果显示,死皮植株胶乳中HbLEA14的表达量明显高于健康树。
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| 关 键 词： | 巴西橡胶树 胚胎晚期丰富蛋白 基因克隆 序列分析 RT-PCR |
Cloning and Sequence Analysis of the Late Embryogenesis Abundant Related Protein
Gene from Hevea brasiliensis |
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| YU Xiudao,WANG Zhenhui,YUAN Kun and YANG Lifu.Cloning and Sequence Analysis of the Late Embryogenesis Abundant Related Protein
Gene from Hevea brasiliensis[J].Chinese Journal of Tropical Crops,2011,32(7):1304-1308. |
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| Authors: | YU Xiudao WANG Zhenhui YUAN Kun and YANG Lifu |
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| Institution: | YU Xiudao,WANG Zhenhui,YUAN Kun,YANG Lifu Rubber Research Institute/Key Laboratory of Rubber Biology,Ministry of Agriculture,CATAS,Danzhou,Hainan,571737,China |
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| Abstract: | Using the primers designed from the known EST from a suppression subtractive hybridization-cDNA library,the full-length cDNA sequence of HbLEA14 was cloned from Hevea brasiliensis latex by 3'-RACE technique.The opening reading frame(ORF)of HbLEA14 was 456 bp in length,encoding a 151-amino-acid polypeptide with a predicted molecular weight of 16.58 ku and isoelectric point of 5.10.Cluster analysis revealed that HbLEA14 was an atypical LEA2,and showed 88% identity with Ricinus communis RcLEA14,80% to Euphorbi... |
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| Keywords: | Hevea brasiliensis LEA protein Gene cloning Sequence analysis RT-PCR |
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