| 测定生姜蛋白酶活性的新方法 |
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| 引用本文： | 于洁,肖超妮,王世祥,郑晓晖.测定生姜蛋白酶活性的新方法[J].热带作物学报,2011,32(8):1475-1478. |
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| 作者姓名： | 于洁 肖超妮 王世祥 郑晓晖 |
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| 作者单位： | 西北大学生命科学学院,陕西西安,710069 |
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| 基金项目： | 陕西省教育厅自然科学项目(09JK788); 陕西省中医管理局09年项目(zy58) |
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| 摘 要： | 通过对生姜蛋白酶进行分离纯化,并将其加热使酶失活作为参照,用考马斯亮蓝染色法测定蛋白酶活力。研究了pH值、温度、EDTA和金属离子(Na+,Mg2+,Cu2+,Ca2+)等因素对生姜蛋白酶活性的影响。结果表明,以失活的生姜蛋白酶为参照,可以简洁的测定出生姜蛋白酶的活性,与已往测定生姜蛋白酶活性相比较,具有方法简单,消除腐蚀危害,可望简洁而环保的测定出生姜蛋白酶的活性。
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| 关 键 词： | 生姜蛋白酶 加热使酶失活 参照 酶活测定 考马斯亮蓝比色法 |
A New Method for Detecting Ginger Protease Activity |
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| YU Jie,XIAO Chaoni,WANG Shixiang and ZHENG Xiaohui.A New Method for Detecting Ginger Protease Activity[J].Chinese Journal of Tropical Crops,2011,32(8):1475-1478. |
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| Authors: | YU Jie XIAO Chaoni WANG Shixiang and ZHENG Xiaohui |
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| Institution: | YU Jie,XIAO Chaoni,WANG Shixiang,ZHENG Xiaohui The college of life science,Northwest University,Xi'an,Shanxi 710069,China |
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| Abstract: | In this paper,a new method for detecting ginger protease activity was proposed by using inactive ginger protease as a reference.Coomassie brilliant blue staining method was employed to detect the activity of ginger protease.The influence of some factors,such as the pH,temperature,metallic ions(Na+,Mg2+,Cu2+,Ca2+)and EDTA on the activity of the protease was discussed.The results indicate that taking the inactive ginger protease as a reference could detect the activity of ginger protease laconically,which exh... |
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| Keywords: | Ginger protease Inactivate the enzyme by heating Reference Determination of enzyme activity Coomassie brilliant blue colorimetric method |
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