| 枯草芽孢杆菌CS16菌株抑菌活性物质的提取分析 |
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| 引用本文： | 张慧,刘永华,林陈强,邱宏端,陈济琛.枯草芽孢杆菌CS16菌株抑菌活性物质的提取分析[J].热带作物学报,2011,32(10):1893-1897. |
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| 作者姓名： | 张慧 刘永华 林陈强 邱宏端 陈济琛 |
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| 作者单位： | 1. 福建省农业科学院土壤肥料研究所微生物室,福建福州,350003
2. 福州大学生物科学与工程学院,福建福州,350108 |
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| 基金项目： | 福建省自然科学基金资助项目(No.2009J01096); 公益类科研院所专项(No.2009R10032-2) |
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| 摘 要： | 枯草芽孢杆菌CS16发酵液经盐酸沉淀,甲醇抽提得到粗提物,稳定性试验结果表明,抑菌物质在pH2～11较稳定,对温度、紫外、有机溶剂耐受力较好.利用中压液相色谱得到较纯的抑菌物质,薄层层析后显色分析表明,该抑菌物质初步可以确定为酚类物质.
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| 关 键 词： | 枯草芽孢杆菌 中压液相 抑菌活性 分离纯化 |
Extraction of Inhibitive Substances Produced by Bacillus subtilis CS16 Strain |
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| ZHANG Hui,LIU Yonghu,LIN Chenqiang,QIU Hongduan and CHEN Jichen.Extraction of Inhibitive Substances Produced by Bacillus subtilis CS16 Strain[J].Chinese Journal of Tropical Crops,2011,32(10):1893-1897. |
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| Authors: | ZHANG Hui LIU Yonghu LIN Chenqiang QIU Hongduan and CHEN Jichen |
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| Institution: | ZHANG Hui1,LIU Yonghua2,LIN Chenqiang1,QIU Hongduan2,CHEN Jichen1 1 The Soil and Fertilizer Institute,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350003,China 2 Biology Science and Engineering College,Fuzhou University,Fujian 350108,China |
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| Abstract: | Liquid culture of Bacillus subtilis CS16 strain was precipitated by HCl,and extracted with mechanol.The crude extract was stable in the treatment of high temperature and ultraviolent,and was also stable in organic solvents and wide pH range of methanol.The crude extract was then purified by medium-pressure liquid chromatography.The thin-layer chromatography analysis indicated that the inhibition substance was polyhenols. |
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| Keywords: | Bacillus subtilis Medium-pressure liquid chromatography Inhibition activity Isolation and purification |
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