| 腊梅花水通道蛋白CpTIP基因甘露糖筛选体系植物表达载体的构建 |
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| 引用本文： | 成瑜,刘昭军,刘丽艳,李铁,赵曦. 腊梅花水通道蛋白CpTIP基因甘露糖筛选体系植物表达载体的构建[J]. 安徽农业科学, 2010, 38(1): 68-69,71. DOI: 10.3969/j.issn.0517-6611.2010.01.028 |
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| 作者姓名： | 成瑜 刘昭军 刘丽艳 李铁 赵曦 |
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| 作者单位： | 黑龙江省农业科学院,黑龙江哈尔滨,150086;黑龙江省农业科学院,黑龙江哈尔滨,150086;黑龙江省农业科学院,黑龙江哈尔滨,150086;黑龙江省农业科学院,黑龙江哈尔滨,150086;黑龙江省农业科学院,黑龙江哈尔滨,150086 |
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| 基金项目： | 农业部转基因植物专项“抗逆转基因大豆新品种的培育”子课题(2008ZX08004-2) |
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| 摘 要： | [目的]构建以甘露糖为筛选剂的抗旱、抗盐碱植物表达载体，进一步选育无标记的抗逆作物品种。[方法]利用腊梅花水通道蛋白CpTIP cDNA和大肠杆菌pmi基因构建植物表达载体，将抗逆基因与甘露糖正向选择系统结合。[结果]成功构建了腊梅花水通道蛋白印肿基因甘露糖筛选体系植物表达载体pPMI：：CpTIP。[结论]所构建的载体结合了抗逆基因和甘露糖正向选择系统的优点，将抗逆基因与环境友好型筛选体系很好的结合起来。
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| 关 键 词： | 水通道蛋白 甘露糖筛选体系 TIP |
Construction of Chimonanthus praecox(L.)Link Aquaporin CpTIP Plant Expression Vector with Mannose Selection System |
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| CHENG Yu et al. Construction of Chimonanthus praecox(L.)Link Aquaporin CpTIP Plant Expression Vector with Mannose Selection System[J]. Journal of Anhui Agricultural Sciences, 2010, 38(1): 68-69,71. DOI: 10.3969/j.issn.0517-6611.2010.01.028 |
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| Authors: | CHENG Yu et al |
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| Affiliation: | Heilongjiang Academy of Agricultural Sciences;Harbin;Heilongjiang 150086 |
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| Abstract: | [Objective] The aim was to construct drought and saline-alkaline resistance plant expression vector with mannose as selective agent, and further breed unmarked resilient varieties. [Method] The plant expression vector was constructed by using Chimonanthus praecox(L.)Link aquaporin CpTIP cDNA and Escherichia coli pmi gene, combined stress resistance gene with mannose positive selection system. [Result] The test successfully constructed the plant expression vector pPMI∶∶CpTIP. [Conclusion] The constructed vector linked advantages of stress resistance gene and mannose positive selection system. |
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| Keywords: | Aquaporin Mannose selection system TIP |
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