| 洋虫超氧化物歧化酶的最佳提取条件 |
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| 引用本文： | 董龙,迟德富,张蓓蓓,李晓灿,宇佳.洋虫超氧化物歧化酶的最佳提取条件[J].东北林业大学学报,2009,37(4). |
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| 作者姓名： | 董龙 迟德富 张蓓蓓 李晓灿 宇佳 |
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| 作者单位： | 东北林业大学,哈尔滨,150040 |
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| 基金项目： | 国家自然科学基金，黑龙江省杰出青年基金 |
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| 摘 要： | 运用单因素和正交试验设计对洋虫超氧化物歧化酶(SOD)的提取工艺进行了优化研究,得到最优的提取条件为:用m(洋虫粉末):V(抽提液)=1 g:13 mL的0.13 mol/L NaCl溶液在28℃下抽提14 h.活性可达38.83U/mL,比活力(以蛋白计)可达123.66 U/mg.结果表明,NaCl溶液比传统的磷酸盐缓冲液抽提效果更好,其抽提浓度对SOD的提取结果影响最大;同时得到SOD的最佳热变性温度为60℃,时间为15 min.
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| 关 键 词： | 洋虫 提取 优化 |
Optimization of Extraction Conditions for Superoxide Dismutase from Martianus dermestoides |
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| Dong Long,Chi Defu,Zhang Beibei,Li Xiaocan,Yu Jia.Optimization of Extraction Conditions for Superoxide Dismutase from Martianus dermestoides[J].Journal of Northeast Forestry University,2009,37(4). |
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| Authors: | Dong Long Chi Defu Zhang Beibei Li Xiaocan Yu Jia |
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| Institution: | Dong Long,Chi Defu,Zhang Beibei,Li Xiaocan,Yu Jia(School of Forestry,Northeast Forestry University,Harbin 150040,P.R.China) |
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| Abstract: | The extraction conditions of superoxide dismutase(SOD) from Martianus dermestoides was optimized by single factor trial and orthogonal experimental design.The optimal extraction conditions for SOD from M.dermestoides were obtained as extraction solution 0.13mol/L NaCl,ratio of M.dermestoides powder to extraction solution 1g:13mL,extraction temperature 28 degrees C,and extraction time 14 hours.The activity of SOD was 38.83U/mL,and the specific enzymatic activity of SOD was 123.66U/mg under the optimal extrac... |
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| Keywords: | SOD |
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