对蚯蚓纤溶酶的性质和纤溶活性的研究 |
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引用本文: | 迟玉杰,田波,张英华. 对蚯蚓纤溶酶的性质和纤溶活性的研究[J]. 东北农业大学学报, 1999, 30(4) |
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作者姓名: | 迟玉杰 田波 张英华 |
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作者单位: | 东北农业大学食品学院,黑龙江哈尔滨,150030 |
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基金项目: | 黑龙江省杰出青年科学基金 |
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摘 要: | 从蚯蚓组织中分离纯化出一种纤溶酶。研究表明,其最适pH值为80,最适温度为57℃,热稳定性较好,金属离子Na+,K+,Mg2+等可提高此酶的活力,而Hg2+,Ca2+等对此酶有一定的抑制作用。采用平板法测定此酶的血纤维蛋白溶解活性,证明此酶具有强烈的纤溶活性。
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关 键 词: | 蚯蚓 纤溶酶 最适温度 最适pH 金属离子 纤溶活性 |
STIUDIES ON PROPERTIES AND THROMBOLYTIC ACTIVITY OF FIBRINOLYTIC ENZYME FROM EARTHWORM |
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Abstract: | Abstract:A fibrinolytic enzyme was isolated and purified from the crude extract of earthworm by biochemical methods.Its optimum pH value is 8 0,the optimum temperature is 57℃ and it is stable to heat.The metal ions Na +,K +,Mg 2+ can increase its activity and Ca 2+ ,Hg 2+ show some inhibition.The thrombolytic activity on fibrin was measured by fibrin plate method.The results show the enzyme has strong thrombolytic action on fibrin.$$$$ |
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Keywords: | earthworm fibrinolytic enzyme optimum temperature optimum pH metal ions thrombolytic activity |
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