蕃拉芦荟水溶性多糖的分离与鉴定

以蕃拉芦荟（ Aloe barbadensis Miller)为材料，从中分离出3种多糖PAB1、PAB2和PAB3。PAB1的总糖含量和糖醛酸含量分别为78.57%和14.59%；PAB2的分别为88.72%和6.90%；PAB3的分别为50.23%和10.9%，蛋白质含量为28.81%。红外光谱、气相色谱、酶水解等测定结果表明，PAB1为葡萄聚糖，PAB2为葡萄甘露聚糖，PAB3是一种含有甘露糖的糖蛋白。

Sturctural Determination Determination of Polysaccharides Isolated from Aloe barbadensis Miller

Three kinds of polysaccharides, PAB1, PAB2 and PAB3,were extracted from Aloe barbadensis Miller. The purity of PAB1, PAB2 and PAB3 was 78.57 %,88.72 %, 50.23 %, and the content of glucuronic acid was 14.59 %, 6.90 % and 10.9% respectively. PAB3 was a glycoprotein with 28.81 % of protein. The result indicated that there might be no linear chain of a but B among the polysaccharides after enzymolysis. Infrared spectrum analysis showed that all had typical absorption peaks of polysaccharide and acetyl. These showed that the three polysaccharides were partially acetylated B pyrone acid het- eropolysaccharides. Gas chromatography analyses revealed that PAB1 was composed of glucose, PAB2 of glucose and mannose, and PAB3 of mannose.