| 花生fw2.2基因的克隆及遗传转化 |
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| 引用本文： | 刘强,王晶珊,乔利仙,赵春梅,隋炯明. 花生fw2.2基因的克隆及遗传转化[J]. 核农学报, 2011, 25(6): 1123-1128,1210 |
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| 作者姓名： | 刘强 王晶珊 乔利仙 赵春梅 隋炯明 |
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| 作者单位： | 青岛农业大学生命科学学院青岛市主要农作物创新与应用重点实验室,山东青岛,266109 |
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| 基金项目： | 国家自然科学基金，山东省自然基金青年基金，山东省中青年科学家奖励基金，青岛农业大学博士启动基金 |
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| 摘 要： | fw2.2基因是影响番茄果重的一个重要数量性状基因,在心皮的细胞分裂中起负调控作用。本研究以番茄fw2.2基因的序列为探针,从花生EST数据库中筛选同源序列。根据花生的EST拼接序列设计引物对花生进行扩增,目标产物测序后,将推测的氨基酸序列与其他植物fw2.2基因编码的氨基酸序列进行比对,同源性为34.78%～66.8...
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| 关 键 词： | 花生 fw2.2基因 克隆 转化 |
| 收稿时间： | 2011-01-19 |
| 修稿时间： | 2011-04-24 |
CLONING AND GENETIC TRANSFORMATION OF fw2.2 GENE IN PEANUT |
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| LIU Qiang,WANG Jing-shan,QIAO Li-xian,ZHAO Chun-mei,SUI Jiong-ming. CLONING AND GENETIC TRANSFORMATION OF fw2.2 GENE IN PEANUT[J]. Acta Agriculturae Nucleatae Sinica, 2011, 25(6): 1123-1128,1210 |
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| Authors: | LIU Qiang WANG Jing-shan QIAO Li-xian ZHAO Chun-mei SUI Jiong-ming |
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| Affiliation: | Qingdao Key Lab of Germplasm Innovation and Application of Major Crops, College of Life Science, Qingdao Agricultural University, Qingdao, Shandong 266109 |
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| Abstract: | fw2.2 gene is an important quantative trait locus (QTL) affecting fruit weight in tomato, and can reduce cell division in carpels as a negative regulator. In this experiment, fw2.2 gene in tomato was prepared as probe to screen homologous sequence from peanut EST database. Then, according to the joint EST sequence, a pair of primer was designed to amplified the fw 2.2 gene of peanut. Compared with other plants, the similarity range of amino acid sequence was between 34.78% and 66.85%. Expression difference was detected between wild and cultivated materials by semi-quantitative RT-PCR. After transformation mediated by Agrobacterium tumefaciens, 12 independent transformants of peanut cultivar Huayu 23 were identificated by PCR amplification. |
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| Keywords: | peanut fw2.2 gene cloning transformation |
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