玉米自交系48-2和R08辐照后代M_3株系遗传变异的SSR分析

为研究玉米自交系辐射诱变后代的性状遗传变异规律,促进突变体的高效选择和利用,本研究用3种剂量梯度的60Coγ射线对玉米自交系48-2和R08的种子进行辐射诱变处理,用32个多态性SSR分子标记对48-2和R08及其辐射诱变后代M3株系等103份材料进行了遗传变异分析。结果表明：48-2和R08 M3株系位点多态信息量（...

SSR MARKER ANALYSIS ON GENETIC VARIATION OF M_3 FROM MAIZE INBRED LINES 48-2 AND R08 AFTER IRRADIATION INDUCEMENT

Analyzing the biological effects and the genetic variations of maize mutagenic progenies is important to facilitate effective selections and utilization of the mutants.In this study, the genetic variation of 103 mutagenic progenies of M3 lines of inbred lines 48-2 and R08 with60Co γ-rays inducement were evaluated with SSR molecular markers.The results indicated that, the amplitude of polymorphism information content (PIC) of the 48-2 and R08 M3 lines ranged 0.307~0.948 and 0.108~0.955,with an average of 0.762 and 0.701,respectively.The amplitude of genetic diversity indexes (H') ranged 0.552~2.830 and 0.254~3.309,with an average of 1.830 and 1.777,respectively.The average value of genetic similarity coefficient of the 49 M3 lines of 48-2 with its check (M673) was 0.8194.However,the average value of genetic similarity coefficient of the M3 lines of R08 with its check (M487) was 0.8373. Based on the genetic similarity coefficient, inbred lines 48-2, R08 and their 101 M3 lines were clustered in 7 and 5 populations respectively.This phenomenon indicated that massive genetic variation could appear in progenies due to irradiation. The strengthen of selection and utilization of mutants based on the breeding objectives and in accordance with the feature and regularity on genetic variations of main characteristics of mutant lines in various populations could be enhanced in breeding program,to some extent,which can increase the breeding efficiency of irradiation induced mutation in maize.