紫外线对杏鲍菇原生质体的诱变作用

对杏鲍菇（Pleurotus eryngii）菌株PL7的原生质体进行了紫外线诱变的研究。结果发现,用20W紫外灯（245nm）,在垂直距灯管30cm处照射90s,PL7菌株的原生质体致死率达74%。获得再生菌株234株,其中20株与亲本菌株PL7有明显的拮抗反应,6个菌株菌落生长速度提高11.0%～17.8%,液体培...

UV-MUTAGENESIS OF PROTOPLASTS OF Pleurotus eryngii

The UV-mutagenesis of protoplast was conducted on Pleurotus eryngii strain PL7. The results showed that the lethality rate of protoplast reached to 74% by using a 20W germicidal lamp with emitting wavelength of 254nm under irradiation condition of vertical distance of 30cm from lamp to culture media for an irradiation duration of 90s. Twenty mutants were selected from 234 regenerating colonies by antagonistic test. 6 isolates exhibiting 11.0%~17.8% increase of colony growth speed, 19.3%~32.4% increase of mycelium biomass in liquid medium, and new Rep-PCR genetypic pattern were screened from the 20 mutants. The assay of mushroom cultivation revealed that the 6 isolates shortened the overgrowth time of mycelium on culture medium by 3~6 days, advanced the appearance time of mushroom anlage by 5~8 days, and increased the mushroom yield of the first harvest by 12.5%~40.6% and the total biological efficiency by 11.1%~32.4%. The results indicated that the protoplast UV-mutagenesis was a better approach for novel high-yield isolate selection and strain improvement of edible fungi.