Shoot multiplication of <Emphasis Type="Italic">Syringa reticulata</Emphasis> var. <Emphasis Type="Italic">mandshurica</Emphasis> from in vitro cultured seedlings |
| |
| Authors: | Chun-ping Liu Hua-ying Liu Bo-wen Yang Ling Yang Peng Zhang Hai-long Shen |
| |
| Institution: | 1.School of Forestry,Northeast Forestry University,Harbin,China;2.State Key Laboratory of Forest Genetics and Breeding,Northeast Forestry University,Harbin,China;3.Academic Journal Publishing Division,Higher Education Press,Beijing,China;4.College of Landscape Architecture,Northeast Forestry University,Harbin,China |
| |
| Abstract: | We developed a shoot multiplication protocol for Syringa reticulata Blume var. mandshurica Hara from in vitro cultured seedlings that derived from in vitro germinated seeds. The shoots could be induced on Murashige and Skoog (MS) medium with proper plant growth regulator combinations of 6-benzylaminopurine (BA) and indole-3-butyric acid (IBA). The better medium for shoot multiplication and growth was MS + 5 mg L?1 BA + 0.5 mg L?1 IBA + 20 g L?1 sucrose + 7 g L?1 agar, and the corresponding shoot induction rate was 75 %. The plantlets grew well after rooting on 1/2MS medium (macro-elements of MS medium are at half-strength) supplemented with 1 mg L?1 IBA, and the survival percentage was >80 % at 16 weeks after transplanting. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
