| 茶树悬浮细胞茶氨酸生物合成动态研究 |
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| 引用本文： | 成浩,高秀清. 茶树悬浮细胞茶氨酸生物合成动态研究[J]. 茶叶科学, 2004, 24(2): 115-118. DOI: 10.13305/j.cnki.jts.2004.02.008 |
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| 作者姓名： | 成浩 高秀清 |
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| 作者单位： | 中国农业科学院茶叶研究所,农业部茶叶化学工程重点开放实验室,浙江 杭州 310008 |
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| 基金项目： | 浙江省自然科学基金，青年科技人才培养专项资助 |
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| 摘 要： | 对不同外植体来源的茶树培养细胞的茶氨酸生物合成能力、适宜培养细胞合成茶氨酸的培养基条件、培养细胞茶氨酸生物合成动态和更新培养基对提高培养细胞茶氨酸累积含量的效果等进行了分析。在一个培养周期中,培养细胞的茶氨酸累积高峰出现在第11βd左右。在以每10βd更新一次培养基的条件下,培养细胞茶氨酸合成能力可维持至第30βd左右,达到细胞干重的近20%。
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| 关 键 词： | 茶树 培养细胞 茶氨酸 生物合成 |
| 文章编号： | 1000-369X(2004)02-0115-04 |
| 收稿时间： | 2004-02-17 |
| 修稿时间： | 2004-02-17 |
Dynamics of Theanine Biosynthesis in Tea Suspension Cells |
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| CHENG Hao,GAO Xiu-qing. Dynamics of Theanine Biosynthesis in Tea Suspension Cells[J]. Journal of Tea Science, 2004, 24(2): 115-118. DOI: 10.13305/j.cnki.jts.2004.02.008 |
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| Authors: | CHENG Hao GAO Xiu-qing |
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| Affiliation: | Tea Research Institute, Chinese Academy of Agricultural Sciences, Key Lab of Tea Chemical Engineering, Ministry of Agriculture, Hangzhou 310008, China |
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| Abstract: | The level of theanine biosynthesis of tea cultured cells initiated from different explant material and the dynamics of theanine biosynthesis in tea suspension cell were analyzed. The suitable medium for theanine biosynthesis and the effect of medium renewal were also tested. According to the result, the peak of theanine accumulation was appeared on the 11th day during a culture cycle. But if the medium was renewed in an interval of 10 days, theanine could be continuously accumulated in the cells until about the 30th day and reached a high peak of 20% cell dry weight. |
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| Keywords: | Tea Cultured cell Theanine Biosynthesis |
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