| 桤叶唐棣初代培养影响因素研究 |
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| 引用本文： | 杜保国,杨锋利,杨途熙,魏安智. 桤叶唐棣初代培养影响因素研究[J]. 北方园艺, 2010, 0(20) |
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| 作者姓名： | 杜保国 杨锋利 杨途熙 魏安智 |
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| 作者单位： | 绵阳师范学院生命科学与技术学院;绵阳师范学院城乡建设与规划学院;西北农林科技大学生命科学学院; |
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| 摘 要： | 为了探索影响桤叶唐棣初代培养无菌苗建立的因素,现以带芽茎段为外植体,研究了不同消毒方式的消毒效果以及基本培养基类型和激素组合对初代培养的影响。结果表明:10%次氯酸钠10 min和0.1%升汞2 min混合使用的消毒方式,取得了较好的消毒效果,污染率和致死率均较低,分别为11.2%和9.5%;MS培养基为较适宜的初代培养,其增殖芽数、茎芽长分别为2.45和1.18 cm;外植体在MS+IAA 0.1 mg/L+6-BA 2.0 mg/L上生长最好。
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| 关 键 词： | 桤叶唐棣 初代培养 影响因素 |
Study on the Influencing Factors of Initial Culture of Amelanchier alnifolia Nutt |
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| DU Bao-guo,YANG Feng-li,YANG Tu-xi,WEI An-zhi. Study on the Influencing Factors of Initial Culture of Amelanchier alnifolia Nutt[J]. Northern Horticulture, 2010, 0(20) |
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| Authors: | DU Bao-guo YANG Feng-li YANG Tu-xi WEI An-zhi |
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| Affiliation: | DU Bao-guo1,YANG Feng-li2,YANG Tu-xi3,WEI An-zhi3(1.College of Life Science and Biotechnology,Mianyang Normal University,Mianyang,Sichuan 621000,2.College of Urban and Rural Development and Planning,3.College of Life Science,Northwest Agriculture and Forestry University,Yangling,Shaanxi 712100) |
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| Abstract: | Stems with buds were used as explants to study how disinfection methods,basic medium and different hormone affect the primary culture of Amelanchier alnifolia Nutt.The results showed that the best disinfection method was using 10% NaClO for 10 minutes and then 0.1%HgCl2 for 2 minutes,it had lower contaminated rate and death rate;MS medium was the best one for primary culture with high multiplication and length of buds,2.45 and 1.18 cm respectively;explants grew well in the medium of MS with IAA 0.1 mg/L and... |
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| Keywords: | Amelanchier alnifolia Nutt initial culture influencing factors |
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