Convenient synthesis of lactuloselysine and its use for LC-MS analysis in milk-like model systems. |
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| Authors: | F Vinale S M Monti B Panunzi V Fogliano |
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| Institution: | Dipartimento di Scienza degli Alimenti, Università degli Studi di Napoli "Federico II", Parco Gussone, 80055 Portici, Napoli, Italy. |
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| Abstract: | The synthesis of the Amadori product lactuloselysine N(epsilon)-(1-deoxy-D-lactulosyl-1)-L-lysine] was obtained starting from FMOC-lysine-OH (N(alpha)-9-fluorenylmethoxy-carbonyl-N(epsilon)H(2)-L-lysine-OH) and lactose. Compound identity was confirmed by MALDI-ToF, electrospray, and NMR analysis. A selective LC-MS procedure which allowed the detection of lactuloselysine up to 10 ng mL(-)(1) was set up and used to follow the formation of the compound in a lactose-lysine model system; quantification of this molecule after complete enzymatic hydrolysis of whey-proteins from milk samples was also performed. |
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