不同时期小鼠睾丸睾酮合成能力差异机制的研究

在不同发育阶段，睾丸合成睾酮的能力差异很大，为了阐明引起类固醇合成差异的分子机制，本实验以不同年龄的SPF昆白小鼠为研究对象，采用RT-PCR,Western Blot分析了不同年龄阶段类固醇合成酶的变化。结果表明：（1）在不同年龄阶段，P450SCCmRNA、3β-HSDmRNA、StAR mRNA和蛋白的水平无明显变化；（2）P450c17mRNA的表达水平在30d、60d、120d均处于较高的水平，但是在270d则处于较低的水平；（3）从30d到270d，ERK活性无明显变化。这表明， ERK活性可能与衰老引起睾酮合成能力下降无关。

Changes of The Ability of Testosterone Synthesis In Different Peroids

To study the mechanism of Changes of the ability of testosterone synthesis in different periods , kunbai mouse of different peroids wereused as experimental animals . the ability of testosterone synthesis was analyzed by RT-PCR and Western Blot.The results were as followed: (1) In all peroids, the expression of P450scc ,3β-HSD，StAR mRNA and protein had no significant change.(2)The expression of P450c17 mRNA had no significant change in 10d,60d,120d.however, The expression of P450c17 mRNA decreased significantly in 180d.(3) From 30d to 270d, the activity of ERK hadno significantchange. These results inferred that activity of ERK had no indirect. relationwith the decrease of P450c17 mRNA and the level of testosterone