枳壳EST-SSR标记的开发

GenBank上已公布的枳壳EST序列为开发新的SSR标记提供了宝贵的数据资源。本研究利用在线SSR鉴定软件SSRIT分析来自枳壳EST数据库的11029条Unigene序列。分析结果共发现327条EST序列含有348个SSR位点,占总数的2.96%。其中,三核苷酸重复的SSR类型最多,共有161个,占检索总数的46.26%。Primer 3.0设计合成58对EST-SSR引物,其中36对能扩增出产物,6对引物产生多态性分离,分别占所设计引物总数的62.07%和10.34%。本文研究成果为今后枳壳遗传多样性分析、遗传图谱构建及比较基因组等研究方面奠定了基础。

Development of EST-SSR Markers in(Citrus aurantium L.)

The big collection of expressed sequence tags(ESTs) from Citrus aurantium L.is available in public database,and offers an opportunity to identify simple sequence repeats(SSR) in ESTs by data mining.These sequences may provide an estimate of diversity in the expressed portion of the genome and may be useful for comparative mapping,for tagging important traits of interest,and for additional map-based cloning of important genes.We analyzed frontal 11 029 Unigene sequences from C.aurantium database using online SSR identified software SSRIT.Totally,327 ESTs with 348 SSRs were identified,and accounted for 2.96% of the total number of EST sequences.Trinucleotide repeats(a total of 161) were the most abundant repeat class,and accounted for 46.26% of all found SSRs.According to these EST sequences containing SSR,58 primer pairs were designed using Primer 3.0.of these,36 primer pairs amplified DNA fragments and 6 primer pairs exhibited polymorphism,account for 62.07% and 10.34% of the total designed 58 pairs.The development of new EST-SSR markers from C.aurantium has potential important implication for genetic analysis and exploitation of genetic resources of C.aurantium and would provide a more direct estimate of functional diversity.