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Production of high quality Ardisia plants by stem tip cuttings
Institution:1. Center for Clinical Molecular Medicine, Children’s Hospital of Chongqing Medical University, Chongqing, China;2. Children’s Hospital of Chongqing Medical University, Division of Pulmonary and Respiratory Diseases, Chongqing, China;3. Ministry of Education Key Laboratory of Child Development Disorders, Chongqing, China;4. Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Chongqing, China;5. Children’s Hospital of Chongqing Medical University, Department of Neonatology, Chongqing, China
Abstract:To produce commercially acceptable Ardisia plants, stem tip cuttings from mature plants were rooted and forced in greenhouses. Ten centimeter long cuttings were either treated with 200 ppm 1-naphthalene acetic acid (NAA) for 2 h, 2000 ppm indole-3-yl-butyric acid (IBA) for 10 s, or 0.5 and 1.0% IBA powder prior to sticking them in the rooting medium. Rooting percentage at 45 days exceeded 76% with 2000 ppm IBA treatment which was a significant increase over non-treated control. Rooted cuttings developed into three types of plants: those forming only vegetative shoots without flowers, those forming reproductive shoots with flowers, and those forming both vegetative and reproductive shoots. The ideal plant produced only vegetative shoots when rooted cuttings were transplanted into pots. About 50% rooted cuttings were forced to finish, producing 31 or 40% of high quality plants when rooted cuttings with vegetative shoots were grown in a greenhouse (GH) at temperatures higher than 21/19 °C (day/night) in 1995 or 21/18 °C GH in 1997, respectively. This method shortened the total production time to less than 2 years as compared to 4 years when starting from seeds.
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