Molecular analysis of genetic stability in micropropagated apple rootstock MM106 |
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| Affiliation: | 1. University Institute of Biotechnology (UIBT), Chandigarh University, Mohali, Punjab, India;2. Department of Biotechnology, Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Solan, Himachal Pradesh, India;3. Plant Biology Section, School of Integrative Plant Science, Cornell University, Ithaca, NY, United States;1. Dpto. de Pomología, Estación Experimental de Aula Dei (CSIC), Apdo. 13034, 50080 Zaragoza, Spain;2. Dpto. de Suelos y Aguas de Riego, Laboratorio Agroambiental. Gobierno de Aragón, Apdo. 727, 50080 Zaragoza, Spain;1. G.B. Pant National Institute of Himalayan Environment, Himachal Regional Centre, Mohal-Kullu 175126, Himachal Pradesh, India;2. Indian Council of Agricultural Research-Indian Agriculture Research Institute, Regional Station, Katrain-Kullu 175129, Himachal Pradesh, India;3. G.B. Pant National Institute of Himalayan Environment, Ladakh Regional Centre, Leh 194101, India;4. CSIR-Institute of Himalayan Bioresource Technology, Post Box No: 6, Palampur 176061, Himachal Pradesh, India;1. Department of Horticultural Science, Faculty of Agriculture Science, University of Guilan, Rasht, P.O. Box: 41635-1314, Iran;2. Department of Genetic Engineering, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Agricultural Research, Education and Extension Organization (AREEO), Iran;3. Ornamental Plant Research Center, Horticultural Science Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Iran;4. Department of Agronomy Plant Breeding, Faculty of Agriculture Science, University of Guilan, Rasht, P.O. Box: 41635-1314, Iran |
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| Abstract: | Random amplified polymorphic DNA (RAPD) markers were used to assess the genetic stability of 10 micropropagated plants regenerated through axillary buds of clonal apple (Malus pumila Mill.) rootstock MM106. Eleven random decamer primers were successfully used to analyse genomic DNA from mother plants and in vitro plant material. A total of 129 scorable fragments were amplified with an average of 11.73 bands per primer. Among them, 99 were monomorphic and 30 were polymorphic with 23.2% polymorphism. Among these 30, 12 were found monomorphic across seven plants and parent. Three plants could be regarded as off-types. Our results show that RAPD markers could be used to detect the genetic similarities and dissimilarities in micropropagated material. |
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