Analyses of pollen-tube growth and biological action of S-RNase in the style of self-compatible Japanese pear |
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| Institution: | 1. Department of Horticulture, Nanjing Agricultural University, Nanjing 210095, China;2. Faculty of Bioresources, Mie University, 1515 Kamihama-cho, Tsu, Mie 514-8507, Japan;1. Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China;2. Experiment Teaching Center of Agricultural Biology, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China;1. Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, United States;2. Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, United States;1. Department of Agricultural Sciences, University of Bologna, Bologna, Italy;2. Department of Life Sciences and Biotechnology, University of Ferrara, Ferrara, Italy;3. Department of Horticulture, Washington State University—Tree Fruit and Extension Research Center, Wenatchee, WA, USA;1. Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601 Japan;2. Horticultural Experiment Station, Yamagata Integrated Agricultural Research Center, Shima-Minami, Sagae 991-0043, Japan;3. Faculty of Agriculture, Yamagata University, Wakabacho, Tsuruoka 997-0052, Japan;1. Department of Nutrition and Food Hygiene, College of Public Health, Zhengzhou University, Zhengzhou 450001, China;2. Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin Institute of Health and Environment Medicine, Tianjin 300050, China;3. Department of Public Health, Southwest Medical University, Luzhou 646000, China |
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| Abstract: | The Japanese pear ‘Osa-Nijisseiki’ (S2S4SM) (SM = stylar-part mutant) is a self-compatible bud mutant that originated from self-incompatible ‘Nijisseiki’ (S2S4). The S4-allele of the pear is deleted and it shows unilateral incompatibility to cultivar with an S2S4 genotype. However, when pollen-tube growth was compared between cross-compatible ‘Osa-Nijisseiki’ × ‘Okusankichi’ (S5S7)], unilateral-compatible ‘Osa-Nijisseiki’ × ‘Kikusui’ (S2S4)], self-compatible (‘Osa-Nijisseiki’ × ‘Osa-Nijisseiki’), and unilateral-incompatible pollination (‘Kikusui’ × ‘Osa-Nijisseiki’), pollen-tube growth clearly showed the following order: cross-compatible > unilateral-compatible > self-compatible > unilateral-incompatible. This indicates that the ‘Osa-Nijisseiki’ style produces specific inhibitor(s) not only to S2- and S4-pollen but also “self-pollen”, because the phenotype of S4SM-pollen is the same as S4-pollen. Stylar protein analysis demonstrated that ‘Osa-Nijisseiki’ produces S2-RNase (RNase associated with S2-allele) together with a small amount of S4-RNase. The purified S4-RNase possessed almost the same inhibitory action on the growth of S4-pollen-tubes in vitro at 1 μg μl?1 as that from original ‘Nijisseiki’. These results suggest that the depressed growth of unilateral-compatible and self-pollen-tubes in ‘Osa-Nijisseiki’ is due to this biologically active S4-RNase. Growth of self-pollen-tubes may also be depressed by inhibitor(s) specific to “self-pollen” unrelated to S-alleles. |
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