Leaf phenolic content of pear cultivars resistant or susceptible to fire blight |
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| Affiliation: | 1. Ege University Ödemiş, Vocational Training School, 35750 Ödemiş, Izmir, Turkey;2. Ege University, Faculty of Agriculture, Department of Horticulture, 35100 Bornova, Izmir, Turkey;1. Department of Pharmaceutical Chemistry and Drug Analysis, Center for Neurosciences (C4N), Vrije Universiteit Brussel (VUB), Laarbeeklaan 103, 1090 Brussels, Belgium;2. Department of Analytical Chemistry and Pharmaceutical Technology, Vrije Universiteit Brussel (VUB), Laarbeeklaan 103, 1090 Brussels, Belgium;3. Laboratory of Instrumental Analysis and Bioelectrochemistry, Université Libre de Bruxelles (ULB), Boulevard du Triomphe, 1050 Brussels, Belgium;1. División de Estudios de Posgrado, Facultad de Química, Universidad Autónoma de Querétaro, Centro Universitario s/n, Colonia las Campanas, 76010 Querétaro, Mexico;2. Facultad de Ciencias Naturales, Universidad Autónoma de Querétaro. Centro Universitario s/n, Colonia las Campanas, Querétaro 76010, Mexico;1. Department of Food Science, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa;2. Department of Horticultural Science, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa;3. ARC Infruitec-Nietvoorbij, Private Bag X5026, Stellenbosch 7599, South Africa;4. HORTGRO Science, P.O. Box 12789, Die Boord 7613, South Africa;1. Department of Nutrition and Food Hygiene, Hubei Key Laboratory of Food Nutrition and Safety, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China;2. Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, China |
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| Abstract: | Young leaf samples were taken from susceptible and resistant pear varieties in June and August for 2 years in 2000 and 2001 and were analyzed for phenolic compounds by using thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) after artificial inoculation with Erwinia amylovora. The distribution of spots in relation to phenolic compounds on TLC plates showed differences in resistant varieties compared to susceptible ones. The number of spots in resistant varieties was generally higher than the other group. Some spots were peculiar for distinguishing resistant and susceptible ones. Similarly, on HPLC dendograms, mean arbutin value was higher in the resistant group than the susceptibles both in 2000 and 2001. Additionally, arbutin content was found to be higher than chlorogenic acid content both in resistant and susceptible varieties. |
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