Protocol for rapid propagation,and to overcome delayed rhizome formation in field established in vitro derived plantlets of Kaempferia galanga L. |
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| Institution: | 1. Department of Botany, University of Calicut, Kerala 673 635, India;2. Department of Biotechnology, University of Calicut, Kerala 673 635, India;1. College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China;2. Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;1. Department of Horticulture, Aristotle University, 54124, Thessaloniki, Greece;2. Forest Research Institute of Thessaloniki, Greek Agricultural Organization‐Dimitra, 57006, Vassilika, Thessaloniki, Greece;3. Agris S.A., Kleidi, 59300, Imathia, Greece;1. Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei 430074, China;2. University of the Chinese Academy of Sciences, Beijing 100039, China;1. Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla Univesity, Hat-Yai, Songkhla, 90112, Thailand;2. Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Prince of Songkla University, Songkhla, 90112, Thailand;3. Excellent Research Laboratory, Phytomedicine and Pharmaceutical Biotechnology Excellence Center, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai, Songkhla, 90112, Thailand;1. Department of Horticulture, Forestry, and Recreation Resources, Kansas State University, Manhattan, KS 66506, United States;2. Department of Plant Pathology, Kansas State University, Manhattan, KS 66506, United States;3. Kansas State University Olathe Horticulture Research and Extension Center, Olathe, KS 66061, United States |
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| Abstract: | Single medium based efficient protocol for rapid propagation, and to overcome the delayed rhizome formation in field established in vitro derived plantlets of Kaempferia galanga L. through in vitro rhizome induction was achieved. MS medium with combination of 8.87 μM N6-benzyladenine (BA), and 2.46 μM indole-3-butyric acid (IBA) induced a mean of 6.2 shoots per explant. Addition of 11.7 μM silver nitrate to 8.87 μM BA and 2.46 μM IBA supplemented medium facilitated the highest number of shoots (mean of 8.3 shoots) as well as roots within 60 days. Subculture of isolated shoots on medium with the same concentration of BA, IBA and silver nitrate increased the number to a mean of 12.1 shoots. Silver nitrate enriched medium developed rhizome at the base of shoots. Increase of sucrose concentration (6–8%) in medium with BA, IBA and silver nitrate favoured the best rhizome development. Ninety five per cent of the plantlets survived in field conditions. The plantlets established in field without in vitro developed rhizome (from medium with BA and IBA) did not form rhizome even at 7 months after transplantation. Instead, they developed tuberous roots only. The plantlets with in vitro developed rhizome (on medium having BA, IBA, silver nitrate, and 6–8% sucrose), and that established from conventional way (through splitting of old rhizome) showed no difference in growth of the rhizome. The present study emphasizes the efficacy of silver nitrate and sucrose to develop rhizome in vitro, which enabled to overcome the delayed development of rhizome, and reduced yield of plantlets established in field without in vitro developed rhizome. |
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