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Agrobacterium-mediated transformation of Musa acuminata cv. “Grand Nain” scalps by vacuum infiltration
Institution:1. Facultad de Ingeniería Química, Universidad Autónoma de Yucatán, Centro de Investigación Científica de Yucatán, Calle 43, No. 130, Colonia Chuburná de Hidalgo, C.P. 97200 Mérida, Yucatán, México;2. Unidad de Biotecnología, Centro de Investigación Científica de Yucatán, Calle 43, No. 130, Colonia Chuburná de Hidalgo, C.P. 97200 Mérida, Yucatán, México;3. Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, Calle 43, No. 130, Colonia Chuburná de Hidalgo, C.P. 97200 Mérida, Yucatán, México;1. Budker Institute of Nuclear Physics, SB RAS, Novosibirsk, 630090, Russia;2. Novosibirsk State University, Novosibirsk, 630090, Russia;3. Novosibirsk State Technical University, Novosibirsk, 630092, Russia;4. University of Sydney, School of Physics, Falkiner High Energy Physics, NSW 2006, Sydney, Australia;5. University of Tokyo, Department of Physics, 7-3-1 Hongo Bunkyo-ku, Tokyo, 113-0033, Japan;1. Department of Bioinformatics and Biotechnology, GC University, Faisalabad, Pakistan;2. Institute of Biological Sciences, Campus Talca, Universidad deTalca, Talca 3465548, Chile;3. Department of Plant Breeding and Genetics, The University of Haripur, 22620 Khyber Pakhtunkhwa, Pakistan;4. Department of Biochemistry, GC University, Faisalabad, Pakistan;1. College of Agriculture, Guangxi University, Nanning 530004, China;2. State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Nanning 530004, China
Abstract:Two Agrobacterium-mediated plant transformation protocols were evaluated for the generation of transgenic banana tissues (Musa acuminata var. Grand Nain). Co-cultivation versus vacuum infiltration of meristematic banana tissues was compared. The binary vector pC2301 was used for the initial transformation and the histochemical detection of GUS. PCR assays demonstrated that the transformation protocol was successful. Infiltrated samples transformed with pCambia 2301 showed a wider GUS response than the co-cultivated tissues. The specific β-glucuronidase activity was also higher in the infiltrated tissues than in co-cultivated ones and was also comparable to that found in other work. The vector BIBAC2 (bearing a 50 kb insert of foreign gDNA) was also transformed with these protocols showing the potential of this procedure on future genomics for this cultivar.
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