Induction of somatic embryogenesis in lotus (Nelumbo nucifera Geartn.) |
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| Institution: | 1. Department of Horticulture, Faculty of Agricultural Technology, King Mongkut''s Institute of Technology, Ladkrabang, Bangkok 10520, Thailand;2. Programme Agriculture, Faculty of Agricultural Technology, Rajabhat Institute Phuket, Phuket 83000, Thailand;1. Pharmaceutical Research Laboratory, Biotechnology Research Department, Ministry of Education, Mandalay Division, Kyaukse 05151, Myanmar;2. Department of Research and Innovation (DRI), Ministry of Education, Yangon 11081, Myanmar;3. Cell Culture Laboratory, Biotechnology Research Department, Ministry of Education, Mandalay Division, Kyaukse 05151, Myanmar;1. Queensland Alliance for Agriculture and Food Innovation, University of Queensland, St Lucia, QLD 4072, Australia;2. Faculty of Science, Education and Engineering, University of the Sunshine Coast, Sippy Downs, QLD 4556, Australia;1. Key Laboratory of Plant Resources and Beijing Botanical Garden, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China;2. University of Chinese Academy of Sciences, Beijing 100049, China;3. Shanghai Chenshan Plant Science Research Center, Chinese Academy of Sciences, Chenshan Botanical Garden, Shanghai 201602, China;1. College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China;2. Wuhan Walksun Biotechnology Limited Company, Wuhan 430000, China;1. National Center for Natural Products Research, School of Pharmacy, Research Institute of Pharmaceutical Sciences, University of Mississippi, University, MS 38677, USA;2. Department of BioMolecular Sciences, School of Pharmacy, Research Institute of Pharmaceutical Sciences, University of Mississippi, University, MS 38677, USA;3. Department of Pharmaceutics and Drug Delivery, School of Pharmacy, Research Institute of Pharmaceutical Sciences, University of Mississippi, University, MS 38677, USA;1. Jiangsu Collaborative Innovation Center of Regional Modern Agriculture & Environmental Protection/Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, Huaiyin Normal University, Huaian 223300, China;2. Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization & Ecological Restoration Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China;3. School of Pharmaceutical Sciences, Tsinghua University, Beijing 100084, China;4. Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China |
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| Abstract: | Callus induction and somatic embryogenesis of lotus (Nelumbo nucifera Gaertn.) cv. Satabankacha were studied. Callus was initiated by culturing bud, cotyledon, and young leaf explants on Murashige and Skoog (MS) (1962) medium containing a combination of 0, 4, 8 and 10 μM 2,4-dichlorophenoxy acetic acid (2,4-D) and 0, 1, 2 and 3 μM 6-furfuryl amino purine (kinetin) or substituting 0, 0.5 and 1 μM benzyladenine (BA) for kinetin. Bud explants cultured on medium containing 4 μM 2,4-D and 1 μM BA gave the best callus growth. For somatic embryogenesis, the calli initiated on MS medium containing a combination of 4, 6, 8 and 10 μM 2,4-D and 1 μM BA and subsequently transferred to media containing 2–4 μM 2,4-D and 0 or 0.5 μM BA produced the most somatic embryos. When cultures were 12-week-old, callus produced on medium with 6 μM 2,4-D and 1 μM BA showed the best growth for somatic embryo regeneration. When transferred to a medium with 2 μM 2,4-D and 0.5 μM BA somatic embryos were produced from 33% of the calli. Embryos developed to the stage proembryo within 4 weeks and formed globular, heart, torpedo and mature embryos within 16 weeks. |
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