Partial vacuum extends the longevity of primed bitter gourd seeds by enhancing their anti-oxidative activities during storage |
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Institution: | 1. Hôpital CarémeauNîmesFrance;2. 24 rue de DoleBesançonFrance;3. 12 rue RiquetParisFrance;1. Department of Pathology, University of Iowa Carver College of Medicine, 200 Hawkins Drive, Iowa City, IA 52242, United States;2. Department of Molecular Physiology and Biophysics, 6-530 Bowen Science Building, University of Iowa Carver College of Medicine, Iowa City, IA 52242;3. Department of Pathology and Laboratory Medicine, Iowa City VA Health System, 601 Highway 6 West, Iowa City, IA 52246, United States |
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Abstract: | This study evaluates the effects of partial vacuum storage on longevity and anti-oxidative responses of primed bitter gourd (Momordica charantia L.) seeds. Priming was achieved by mixing the seeds with moist vermiculite 25 °C for 36 h, followed by air-drying to the original moisture level. Primed seeds were vacuum-packed and stored at 25 °C for up to 12 months. Priming improved seed germination, reduced lipid peroxidation and enhanced anti-oxidative activity prior to storage. However, primed seeds accumulated more total peroxide than non-primed control after 12 months non-vacuum storage, and this led to a marked decrease in seed longevity. Increased total peroxide levels were associated with decreased percentage of 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) radical inhibition. Partial vacuum storage proved useful in extending the longevity of primed seeds for up to 12 months. Improved longevity was related to enhanced anti-oxidative activity that minimized the accumulation of total peroxide during long-term storage. |
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